HIV-1 vectors do not require accessory proteins for efficient transduction of lymphoid cell lines
| . | Percentage of EGFP-positive cells . | ||
|---|---|---|---|
| SupT-1 . | Jurkat . | EBV-B cells . | |
| +APs | 87.0 ± 4.3 | 81.7 ± 2.3 | 90.3 ± 3.2 |
| ΔAPs | 82.6 ± 4.4 | 76.2 ± 4.0 | 89.0 ± 4.2 |
| . | Percentage of EGFP-positive cells . | ||
|---|---|---|---|
| SupT-1 . | Jurkat . | EBV-B cells . | |
| +APs | 87.0 ± 4.3 | 81.7 ± 2.3 | 90.3 ± 3.2 |
| ΔAPs | 82.6 ± 4.4 | 76.2 ± 4.0 | 89.0 ± 4.2 |
Cells were transduced with VSV-G pseudotyped pHR′GK-EGFP vector generated in the presence (+) or the absence (Δ) of HIV-1 accessory proteins and at an MOI of 5 to 10. Stable transgene expression was determined by FACS analysis of EGFP positivity in cells cultured for at least 6 months. Data shown are mean ± SE obtained from 5 independent experiments for SupT-1 and EBV-B cells, and replicate assays using Jurkat cells.