Catalytic efficiencies of the FXIII 34Val and 34Leu activation peptide release by thrombin in the presence or absence of fibrin (FI) or Gly-Pro-Arg-Pro-amide (GPRP)
| kcal/Km (μmol/L)−1 × sec−1 . | Time courses . | Dose response . | Mean ± SD (n = 4) . | +FI +GPRP (n = 3) . | +FI −GPRP (n = 4) . | ||
|---|---|---|---|---|---|---|---|
| 4.0 μmol/L . | 2.0 μmol/L . | 1.0 μmol/L . | |||||
| Valine 34 | 0.22 | 0.20 | 0.17 | 0.21 | 0.20 ± 0.02 | 0.40 ± 0.06 | 2.15 ± 0.13 |
| Leucine 34 | 0.56 | 0.41 | 0.40 | 0.57 | 0.49 ± 0.09 | 0.65 ± 0.11 | 4.81 ± 0.31 |
| kcal/Km (μmol/L)−1 × sec−1 . | Time courses . | Dose response . | Mean ± SD (n = 4) . | +FI +GPRP (n = 3) . | +FI −GPRP (n = 4) . | ||
|---|---|---|---|---|---|---|---|
| 4.0 μmol/L . | 2.0 μmol/L . | 1.0 μmol/L . | |||||
| Valine 34 | 0.22 | 0.20 | 0.17 | 0.21 | 0.20 ± 0.02 | 0.40 ± 0.06 | 2.15 ± 0.13 |
| Leucine 34 | 0.56 | 0.41 | 0.40 | 0.57 | 0.49 ± 0.09 | 0.65 ± 0.11 | 4.81 ± 0.31 |