The effect of SS2 administration on T-cell activity in vivo
| . | Mitogen activity CPM/well . | Flow cytometry % fluorescent positive cells . | |||
|---|---|---|---|---|---|
| ConA . | LPS . | CD4 . | CD8 . | CD19 . | |
| Untreated mouse | 13,921 ± 1,879 | 12,453 ± 806 | 22 ± 1 | 15 ± 1 | 56 ± 1 |
| SS2-treated | 3,131 ± 1,224 | 10,723 ± 1,244 | 1 ± 1 | 4 ± 2 | 63 ± 8 |
| % Change in activity | −77.5%* | −13.9% | −95.6% | −78.9% | +12.5% |
| P value | .002 | NS | .001 | .001 | NS |
| . | Mitogen activity CPM/well . | Flow cytometry % fluorescent positive cells . | |||
|---|---|---|---|---|---|
| ConA . | LPS . | CD4 . | CD8 . | CD19 . | |
| Untreated mouse | 13,921 ± 1,879 | 12,453 ± 806 | 22 ± 1 | 15 ± 1 | 56 ± 1 |
| SS2-treated | 3,131 ± 1,224 | 10,723 ± 1,244 | 1 ± 1 | 4 ± 2 | 63 ± 8 |
| % Change in activity | −77.5%* | −13.9% | −95.6% | −78.9% | +12.5% |
| P value | .002 | NS | .001 | .001 | NS |
Mice (n = 3-4 per group) were given intraperitoneally injections of SS2 (80 g/d) twice daily day 0 to 3. On day 4, splenocytes from treated or untreated control mice were tested for their ability to respond to mitogens conA and LPS using a thymidine incorporation assay. Spontaneous activity (activity in cells not stimulated with mitogen) was 1,260 ± 355 cpm. A sample of the same cells were also tested for the expression of various cell surface markers by flow cytometry as described.