Primers used for RT-PCR analyses
| Fusion gene target . | Primer name and sequence . |
|---|---|
| RARα-PLZF(3′ PLZFbreakpoint) | PLZF-R1 (external): 5′-CTCTTGAGTGTGCTCTCATC-3′ |
| PLZF-R2 (internal): 5′-CTTCCCACACAGCAGACAGA-3′ | |
| NPM-RARα | NPM-F1 (external): 5′-GGAAGATTCGATGGACATGG-3′ |
| NPM-F2 (internal): 5′-TCGGTTGTGAACTAAAGGCC-3′ | |
| RARα-NPM | NPM-R1 (external): 5′-TCTGTGGAACCTTGCTACCA-3′ |
| NPM-R2 (internal): 5′-TGACTCTGCATCTTCCTCCA-3′ | |
| STAT5b-RARα | STAT5b-StgU (external):*5′-GTTTGACGGTGTGATGGAAGTG-3′ |
| STAT5b-F2 (internal): 5′-CAAGCCTCATTGGAATGATG-3′ |
| Fusion gene target . | Primer name and sequence . |
|---|---|
| RARα-PLZF(3′ PLZFbreakpoint) | PLZF-R1 (external): 5′-CTCTTGAGTGTGCTCTCATC-3′ |
| PLZF-R2 (internal): 5′-CTTCCCACACAGCAGACAGA-3′ | |
| NPM-RARα | NPM-F1 (external): 5′-GGAAGATTCGATGGACATGG-3′ |
| NPM-F2 (internal): 5′-TCGGTTGTGAACTAAAGGCC-3′ | |
| RARα-NPM | NPM-R1 (external): 5′-TCTGTGGAACCTTGCTACCA-3′ |
| NPM-R2 (internal): 5′-TGACTCTGCATCTTCCTCCA-3′ | |
| STAT5b-RARα | STAT5b-StgU (external):*5′-GTTTGACGGTGTGATGGAAGTG-3′ |
| STAT5b-F2 (internal): 5′-CAAGCCTCATTGGAATGATG-3′ |
Primer derived from Arnould et al.35