CYA pretreatment permits engraftment of B10.A bone marrow cells in B6 mice conditioned without thymic irradiation or a second mAb injection
| Group* . | Day mAbs administered . | CYA† . | Cortisone‡ . | Fraction of chimeric animals (mean % donor cells + SD)1-153 . | ||||
|---|---|---|---|---|---|---|---|---|
| 2 weeks . | 4 weeks . | 6 weeks . | 8 weeks . | 22 weeks . | ||||
| A | −1, −6 | − | − | 5/5 | 5/5 | 4/5 | 4/5 | 2/3 |
| (25 ± 2) | (32 ± 6) | (21 ± 16) | (23 ± 18) | (56 ± 18) | ||||
| B | −5 | − | − | 4/5 | 4/5 | 4/5 | 2/5 | 0/5 |
| (13 ± 5) | (25 ± 7) | (30 ± 15) | (19 ± 8) | (0) | ||||
| C1-155 | −5 | + | − | 10/10 | 9/9 | 9/9 | 9/9 | 9/9 |
| (12 ± 2) | (41 ± 5) | (48 ± 7) | (55 ± 6) | (70 ± 11) | ||||
| D | −5 | − | + | 3/5 | 1/5 | 1/5 | 1/5 | 1/5 |
| (8 ± 4) | (64) | (63) | (63) | (47) | ||||
| E | −5 | + | + | 3/4 | 2/4 | 1/4 | 1/4 | 1/4 |
| (12 ± 6) | (11 ± 17) | (35) | (31) | (27) | ||||
| Group* . | Day mAbs administered . | CYA† . | Cortisone‡ . | Fraction of chimeric animals (mean % donor cells + SD)1-153 . | ||||
|---|---|---|---|---|---|---|---|---|
| 2 weeks . | 4 weeks . | 6 weeks . | 8 weeks . | 22 weeks . | ||||
| A | −1, −6 | − | − | 5/5 | 5/5 | 4/5 | 4/5 | 2/3 |
| (25 ± 2) | (32 ± 6) | (21 ± 16) | (23 ± 18) | (56 ± 18) | ||||
| B | −5 | − | − | 4/5 | 4/5 | 4/5 | 2/5 | 0/5 |
| (13 ± 5) | (25 ± 7) | (30 ± 15) | (19 ± 8) | (0) | ||||
| C1-155 | −5 | + | − | 10/10 | 9/9 | 9/9 | 9/9 | 9/9 |
| (12 ± 2) | (41 ± 5) | (48 ± 7) | (55 ± 6) | (70 ± 11) | ||||
| D | −5 | − | + | 3/5 | 1/5 | 1/5 | 1/5 | 1/5 |
| (8 ± 4) | (64) | (63) | (63) | (47) | ||||
| E | −5 | + | + | 3/4 | 2/4 | 1/4 | 1/4 | 1/4 |
| (12 ± 6) | (11 ± 17) | (35) | (31) | (27) | ||||
CYA indicates Cyclosporine A; mAb, monoclonal antibody.
Each group received 3 Gy of whole body irradiation on day 0.
CYA given as described, from day −15 to day −3.
Cortisone was given as described, on day 0.
Animals defined as chimeric if level of donor class I+cells in white blood cells was above 2%. Mean percentage of donor class I+ cells in chimeric animals.
Animals pooled from 2 experiments.