Table 1. IGH gene configuration in MM... | American Society of Hematology

Table 1.

IGH gene configuration in MM patients based on Southern blotting, heteroduplex PCR analysis, and sequencing

Patients	MM-1	MM-2	MM-3	MM-4	MM-5	MM-6	MM-7	MM-8	MM-9	MM-10	MM-11	MM-12
MM phenotype	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igλ⁺	True nonproducer	True nonproducer	True nonproducer
Tumor load (cell sample)	35% (BM)	25% (BM)	20% (BM)	90% (BM)	80% (BM)	80% (BM)	30% (BM)	80% (PB)	60% (BM)	25% (skin)	70% (tonsil)	35% (BM)
Southern blotting^*
Jh region	R/G	R/G	R/D	R/D	R/D	R/G	R/G or D	D/D	R/G	R/G or D	R/R	R/D
Cμ	R^NC/G	D/G	R^C/G	R^C/D	D/G	R^C/G	R^C/G	D/G	R^NC/G	R^C/G	D/D	D/G
Cγ^†	G	D/G	G	G	D/G	G	G	G	G	G	G	G
Cα	G	R^C/G	G	G	R^NC/G	G	G	R/G	G	G	R^NC/G	R^C/G
Cε	G	G	R^NC/G	G	D/G	G	G	G	G	G	D/G	G
Heteroduplex PCR and sequencing
Gene segments	V_H1-24/J_H3	negative	ND	D_H3-16/J_H4	D_H2-2/J_H4	D_H2-2/J_H5	D_H4-23/J_H6	NA	V_H3-11/J_H5	D_H1-26/J_H5	V_H2-5/J_H4	V_H1-18/J_H4
Reading frame	+	NA	ND	NA	NA	NA	NA	NA	+	NA	−	+
Somatic hypermutation	+	NA	ND	−	−	−	−	NA	+	−	−	+

Patients	MM-1	MM-2	MM-3	MM-4	MM-5	MM-6	MM-7	MM-8	MM-9	MM-10	MM-11	MM-12
MM phenotype	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igλ⁺	True nonproducer	True nonproducer	True nonproducer
Tumor load (cell sample)	35% (BM)	25% (BM)	20% (BM)	90% (BM)	80% (BM)	80% (BM)	30% (BM)	80% (PB)	60% (BM)	25% (skin)	70% (tonsil)	35% (BM)
Southern blotting^*
Jh region	R/G	R/G	R/D	R/D	R/D	R/G	R/G or D	D/D	R/G	R/G or D	R/R	R/D
Cμ	R^NC/G	D/G	R^C/G	R^C/D	D/G	R^C/G	R^C/G	D/G	R^NC/G	R^C/G	D/D	D/G
Cγ^†	G	D/G	G	G	D/G	G	G	G	G	G	G	G
Cα	G	R^C/G	G	G	R^NC/G	G	G	R/G	G	G	R^NC/G	R^C/G
Cε	G	G	R^NC/G	G	D/G	G	G	G	G	G	D/G	G
Heteroduplex PCR and sequencing
Gene segments	V_H1-24/J_H3	negative	ND	D_H3-16/J_H4	D_H2-2/J_H4	D_H2-2/J_H5	D_H4-23/J_H6	NA	V_H3-11/J_H5	D_H1-26/J_H5	V_H2-5/J_H4	V_H1-18/J_H4
Reading frame	+	NA	ND	NA	NA	NA	NA	NA	+	NA	−	+
Somatic hypermutation	+	NA	ND	−	−	−	−	NA	+	−	−	+

Gene configuration: G indicates germline allele or no rearrangements detectable; R, rearranged allele, R^C, rearranged C_H band, which comigrates with the rearranged J_H band; R^NC, rearranged C_H band, which does not comigrate with the rearranged J_H band; D, deleted allele.

†

It was not possible to determine the configuration of the Cγ gene regions, due to the complexity of the banding patterns (see text).

ND indicates not determined; NA, not applicable.

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