Effect of Cx43 deficiency on B lymphopoiesis
| Genotype . | Embryos . | Neonates . | Young adults . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Fetal liver . | Bone marrow . | Spleen . | Bone marrow . | ||||||||
| Cells × 105 . | CD45R+ sIgM− . | Cells × 105 . | CD45R+ sIgM− . | sIgM+ . | Cells × 105 . | CD45R+ sIgM− . | sIgM+ . | Cells × 106 . | CD45R+ sIgM− . | sIgM+ . | |
| Cx43+/+ | 126.0 ± 26.9* | 6.7 ± 1.0#‡ | 7.9 ± 3.9* | 9.2 ± 0.6 | 2.1 ± 0.6 | 8.9 ± 4.6*‡ | 10.1 ± 0.8 | 12.8 ± 4.9‡ | 8.2 ± 2.3 | 12.6 ± 0.9* | 6.6 ± 1.5 |
| Cx43+/− | 105.9 ± 14.3* | 14.6 ± 7.0# | 3.6 ± 1.5* | 11.3 ± 2.7 | 1.2 ± 0.5 | 4.8 ± 3.8* | 12.9 ± 3.2 | 7.6 ± 5.6 | 9.5 ± 1.0 | 9.2 ± 2.6* | 6.9 ± 2.7 |
| Cx43−/− | 119.8 ± 44.5 | 18.2 ± 8.0‡ | ND | ND | ND | 3.5 ± 0.8‡ | 11.2 ± 7.4 | 3.6 ± 0.8‡ | ND | ND | ND |
| Genotype . | Embryos . | Neonates . | Young adults . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Fetal liver . | Bone marrow . | Spleen . | Bone marrow . | ||||||||
| Cells × 105 . | CD45R+ sIgM− . | Cells × 105 . | CD45R+ sIgM− . | sIgM+ . | Cells × 105 . | CD45R+ sIgM− . | sIgM+ . | Cells × 106 . | CD45R+ sIgM− . | sIgM+ . | |
| Cx43+/+ | 126.0 ± 26.9* | 6.7 ± 1.0#‡ | 7.9 ± 3.9* | 9.2 ± 0.6 | 2.1 ± 0.6 | 8.9 ± 4.6*‡ | 10.1 ± 0.8 | 12.8 ± 4.9‡ | 8.2 ± 2.3 | 12.6 ± 0.9* | 6.6 ± 1.5 |
| Cx43+/− | 105.9 ± 14.3* | 14.6 ± 7.0# | 3.6 ± 1.5* | 11.3 ± 2.7 | 1.2 ± 0.5 | 4.8 ± 3.8* | 12.9 ± 3.2 | 7.6 ± 5.6 | 9.5 ± 1.0 | 9.2 ± 2.6* | 6.9 ± 2.7 |
| Cx43−/− | 119.8 ± 44.5 | 18.2 ± 8.0‡ | ND | ND | ND | 3.5 ± 0.8‡ | 11.2 ± 7.4 | 3.6 ± 0.8‡ | ND | ND | ND |
Cell numbers and frequency values significantly different at: (*)P < .05, (
) P < .01 and (#) P < .025. ND indicates no data.
The numbers of animals per experimental group were as follows: embryos: Cx43+/+ (n = 4), Cx43+/− (n = 12) and Cx43−/− (n = 6); neonates: Cx43+/+ (n = 6), Cx43+/− (n = 5) and Cx43−/− (n = 3); and young adults: Cx43+/+ (n = 4) and Cx43+/− (n = 4).