Table 4. Lptn differentially regulates... | American Society of Hematology

Table 4.

Lptn differentially regulates IL-2 but not IL-4 secretion in DC4⁺ and CD8⁺ T-cell subsets

Stimulus	CD4+ subset^4-150		CD8+ subset^4-150
Stimulus	IL-2 (day 1)	IL-4 (day 3)	IL-2 (day 1)	IL-4 (day 3)
CD3	0.44	0.75	0.18	0.87
CD3 + Lptn	0.09	0.75	0.3	0.95
CD3 + Lptn + anti-Lptn	0.39	0.74	0.21	0.84
CD3 + RANTES	0.44	0.67	0.21	0.88
CD3 + RANTES + anti-RANTES	0.38	0.63	0.18	0.93
CD3 + CD28	2.76	0.45	2.6	0.79

Stimulus	CD4+ subset^4-150		CD8+ subset^4-150
Stimulus	IL-2 (day 1)	IL-4 (day 3)	IL-2 (day 1)	IL-4 (day 3)
CD3	0.44	0.75	0.18	0.87
CD3 + Lptn	0.09	0.75	0.3	0.95
CD3 + Lptn + anti-Lptn	0.39	0.74	0.21	0.84
CD3 + RANTES	0.44	0.67	0.21	0.88
CD3 + RANTES + anti-RANTES	0.38	0.63	0.18	0.93
CD3 + CD28	2.76	0.45	2.6	0.79

The CD4⁺ and CD8⁺ T-cell subsets were purified before activation. They were then activated with the indicated stimulus for the indicated times, after which supernatants were collected and analyzed for IL-2 and IL-4 contents by ELISA, as stated in “Materials and methods.”

F4-150

Data are expressed as concentration mean values in ng/mL and are representative of 4 and 3 independent experiments for the CD4⁺ and CD8⁺ T-cell subsets, respectively, except for the conditions using RANTES, where 2 experiments were done for both subsets.

View Large

This Feature Is Available To Subscribers Only