Exogenous IL-2 overcomes the Lptn-mediated inhibition of CD4+ T-cell proliferation
| Stimulus . | [3H]-Thymidine uptake (cpm)* . |
|---|---|
| CD3 | 13.6 (4) |
| CD3 + IL-2 | 17.2 (6) |
| CD3 + anti-IL-2Rα | 4.9 (4) |
| CD3 + Lptn | 6.6 (2) |
| CD3 + Lptn + IL-2 | 16.2 (7) |
| CD3 + Lptn + anti-IL-2Rα | 9.9 (4) |
| CD3 + CD28 | 30 (13) |
| Stimulus . | [3H]-Thymidine uptake (cpm)* . |
|---|---|
| CD3 | 13.6 (4) |
| CD3 + IL-2 | 17.2 (6) |
| CD3 + anti-IL-2Rα | 4.9 (4) |
| CD3 + Lptn | 6.6 (2) |
| CD3 + Lptn + IL-2 | 16.2 (7) |
| CD3 + Lptn + anti-IL-2Rα | 9.9 (4) |
| CD3 + CD28 | 30 (13) |
The CD4+ T cells were purified, activated for 4 days with the indicated stimulus, before an overnight pulse with [3H]-thymidine, as described in “Materials and methods.”
Numbers represent the mean values of cpm, with SD given in parentheses, obtained from triplicate cultures. The data are representative of 4 experiments performed with different donors.