Gene identities and expression patterns of cDNA fragments cloned by differential display
| cDNA fragment* . | Identity/homology† . | Differential expression in‡ . | Transcript size (kb)1-153 . | ||||
|---|---|---|---|---|---|---|---|
| AML1-MTG8 . | Δ538 . | Δ487 . | AML1b . | AML1a . | |||
| A6C390, I9C440 | MRP14 (M83219) | ↑ | ↓ | → | ↓ | ↑ | 0.6 |
| A6C520 | Unknown | ↑ | ↑ | → | ↓ | ↑ | ND |
| B6G440, D7T390 | Stefin 3 (M92419) | ↑ | → | → | ↓ | ↑ | 0.4 |
| B15T180 | Human Grb10 (D86962) | ↑ | → | → | → | → | 5.0 |
| B16G120 | Unknown | ↑ | ↑ | → | ↓ | ↑ | 3.0 |
| B16G290, L1C1300, L5C1200, P15C280 | Mast cell carboxypeptidase A (J05118) | ↑ | ↑ | → | → | ↑ | 1.4 |
| B16G340, G8T730, H9G390, I6G700, I11G470, I18T1000/950, I20G800/700/440, J3A700, J7G400/290, K13T290, K20A340, K20T220, L16G550, R10G1000, S17T1000 | Granzyme B (X04072) | ↓ | ↓ | → | ↑ | ↓ | 1.6 |
| C3G950 | Unknown | ↑ | ↑ | → | → | → | 2.1 |
| C18A360 | Unknown | ↑ | ↑ | → | → | → | ND |
| G9T200 | Rat β-galactoside-α2,6-syalyltransferase (M83143) | ↓ | ↓ | → | → | → | ND |
| G9T360 | Unknown | ↑ | ↑ | → | → | → | 2.0 |
| G10C360 | Uridine phosphorylase (D44464) | ↑ | ↑ | → | ↓ | ↑ | 1.4 |
| G19T460 | Unknown | ↑ | ↑ | → | → | → | ND |
| I5G520, L1C480, M18G800/200 | Human monocyte/neutrophil elastase inhibitor (M93056) | ↑ | ↑ | → | → | → | 1.5 |
| I19C700/600 | CTLA-2-β (X15592) | ↑ | ↑ | → | → | → | 0.9 |
| J6G550, J11T470 | Rat interferon-induced mRNA (X61381) | ↓ | ↓ | → | → | → | 0.9 |
| J13T600 | Bovine aspartyl-β-hydroxylase (M91213) | ↑ | ↑ | → | → | → | 3.1 |
| J15G700 | Unknown | ↑ | ↑ | → | → | → | >10 |
| K6A220, O4T230 | TIS11b (M58566) | ↑ | ↑ | → | → | → | 2.7 |
| M3A230 | Pim-2 (L41495) | ↓ | ↓ | → | → | ↓ | 2.0 |
| N9T310 | Unknown | ↑ | ↑ | → | → | → | ND |
| P10A370 | Unknown | ↓ | ↓ | → | → | ↓ | ND |
| P13C350 | MIP1α receptor like 1 gene (U28405) | ↑ | ↑ | → | → | → | ND |
| S14T480 | Rat CD53 (M57276) | ↑ | ↑ | → | → | ↑ | 1.7 |
| cDNA fragment* . | Identity/homology† . | Differential expression in‡ . | Transcript size (kb)1-153 . | ||||
|---|---|---|---|---|---|---|---|
| AML1-MTG8 . | Δ538 . | Δ487 . | AML1b . | AML1a . | |||
| A6C390, I9C440 | MRP14 (M83219) | ↑ | ↓ | → | ↓ | ↑ | 0.6 |
| A6C520 | Unknown | ↑ | ↑ | → | ↓ | ↑ | ND |
| B6G440, D7T390 | Stefin 3 (M92419) | ↑ | → | → | ↓ | ↑ | 0.4 |
| B15T180 | Human Grb10 (D86962) | ↑ | → | → | → | → | 5.0 |
| B16G120 | Unknown | ↑ | ↑ | → | ↓ | ↑ | 3.0 |
| B16G290, L1C1300, L5C1200, P15C280 | Mast cell carboxypeptidase A (J05118) | ↑ | ↑ | → | → | ↑ | 1.4 |
| B16G340, G8T730, H9G390, I6G700, I11G470, I18T1000/950, I20G800/700/440, J3A700, J7G400/290, K13T290, K20A340, K20T220, L16G550, R10G1000, S17T1000 | Granzyme B (X04072) | ↓ | ↓ | → | ↑ | ↓ | 1.6 |
| C3G950 | Unknown | ↑ | ↑ | → | → | → | 2.1 |
| C18A360 | Unknown | ↑ | ↑ | → | → | → | ND |
| G9T200 | Rat β-galactoside-α2,6-syalyltransferase (M83143) | ↓ | ↓ | → | → | → | ND |
| G9T360 | Unknown | ↑ | ↑ | → | → | → | 2.0 |
| G10C360 | Uridine phosphorylase (D44464) | ↑ | ↑ | → | ↓ | ↑ | 1.4 |
| G19T460 | Unknown | ↑ | ↑ | → | → | → | ND |
| I5G520, L1C480, M18G800/200 | Human monocyte/neutrophil elastase inhibitor (M93056) | ↑ | ↑ | → | → | → | 1.5 |
| I19C700/600 | CTLA-2-β (X15592) | ↑ | ↑ | → | → | → | 0.9 |
| J6G550, J11T470 | Rat interferon-induced mRNA (X61381) | ↓ | ↓ | → | → | → | 0.9 |
| J13T600 | Bovine aspartyl-β-hydroxylase (M91213) | ↑ | ↑ | → | → | → | 3.1 |
| J15G700 | Unknown | ↑ | ↑ | → | → | → | >10 |
| K6A220, O4T230 | TIS11b (M58566) | ↑ | ↑ | → | → | → | 2.7 |
| M3A230 | Pim-2 (L41495) | ↓ | ↓ | → | → | ↓ | 2.0 |
| N9T310 | Unknown | ↑ | ↑ | → | → | → | ND |
| P10A370 | Unknown | ↓ | ↓ | → | → | ↓ | ND |
| P13C350 | MIP1α receptor like 1 gene (U28405) | ↑ | ↑ | → | → | → | ND |
| S14T480 | Rat CD53 (M57276) | ↑ | ↑ | → | → | ↑ | 1.7 |
cDNA fragment designation was coded from its arbitrary 10-mer, anchor primer, and band size (eg, S14T480 is a 480-bp fragment amplified with S14 10-mer and GT15MT oligo-dT primer).
The origin of each fragment was identified or presumed from the highest scoring sequence in BLAST search of GenBank/EMBL/DDBJ database. The accession number of the highest scoring sequence is also shown in parenthesis.
Arrows pointing up indicate that the differential display band was more intense in the AML1-MTG8, Δ538, Δ487, AML1b, or AML1a lanes than in the control lane, and arrows pointing down indicate the reverse. Arrows pointing right indicate the invariable change by visual inspection.
The transcript size of the major species was determined by Northern blotting analysis. ND indicates not detectable.