Table 3. Binding of multimeric or native... | American Society of Hematology

Table 3.

Binding of multimeric or native VN to immobilized kininogen

Additives	Multimeric VN binding to
Additives	HK	HKa	D5
None	0.058 ± 0.007	0.087 ± 0.006	0.238 ± 0.001
Heparin	0.033 ± 0.001	0.001 ± 0.002	0.002 ± 0.001
Zn²⁺	0.075 ± 0.002	0.701 ± 0.067	0.935 ± 0.088
Zn²⁺ plus heparin	0.066 ± 0.002	0.021 ± 0.007	0.003 ± 0.009

Additives	Native VN binding to
Additives	HK	HKa	D5
None	0.046 ± 0.006	0.082 ± 0.005	0.149 ± 0.004
Heparin	0.047 ± 0.004	0.018 ± 0.012	0.057 ± 0.004
Zn²⁺	0.065 ± 0.022	0.349 ± 0.001	0.308 ± 0.021
Zn²⁺ plus heparin	0.074 ± 0.019	0.204 ± 0.010	0.181 ± 0.012

Additives	Multimeric VN binding to
Additives	HK	HKa	D5
None	0.058 ± 0.007	0.087 ± 0.006	0.238 ± 0.001
Heparin	0.033 ± 0.001	0.001 ± 0.002	0.002 ± 0.001
Zn²⁺	0.075 ± 0.002	0.701 ± 0.067	0.935 ± 0.088
Zn²⁺ plus heparin	0.066 ± 0.002	0.021 ± 0.007	0.003 ± 0.009

Additives	Native VN binding to
Additives	HK	HKa	D5
None	0.046 ± 0.006	0.082 ± 0.005	0.149 ± 0.004
Heparin	0.047 ± 0.004	0.018 ± 0.012	0.057 ± 0.004
Zn²⁺	0.065 ± 0.022	0.349 ± 0.001	0.308 ± 0.021
Zn²⁺ plus heparin	0.074 ± 0.019	0.204 ± 0.010	0.181 ± 0.012

The binding of 2 μg/mL multimeric or native VN to immobilized HKa or D5 (each 5 μg/mL) is shown. Binding was studied in the absence or presence of Zn²⁺ (50 μmol/L) and heparin (10 μg/mL). Binding of multimeric VN to BSA in the absence or presence of Zn²⁺ was 0.163 ± 0.014 and 0.132 ± 0.006, respectively. Binding of native VN to BSA in the absence or presence of Zn²⁺ was 0.143 ± 0.005 and 0.142 ± 0.003, respectively. This nonspecific binding was subtracted to estimate specific binding. Binding is expressed as absorbance at 405 nm. Data are mean ± SEM (n = 3) of a typical experiment, and similar results were obtained in 3 separate experiments.

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