Table 2. Binding of HK or HKa to... | American Society of Hematology

Table 2.

Binding of HK or HKa to immobilized multimeric or native VN

Additives	HK binding to
Additives	VN multimeric	VN native
None	0.024 ± 0.030	0.002 ± 0.001
Heparin	0.005 ± 0.013	0.001 ± 0.001
Zn²⁺	0.025 ± 0.012	0.026 ± 0.033
Zn²⁺ plus heparin	0.001 ± 0.016	0.003 ± 0.007

Additives	HKa binding to
Additives	VN multimeric	VN native
None	0.023 ± 0.011	0.041 ± 0.021
Heparin	0.001 ± 0.030	0.008 ± 0.013
Zn²⁺	0.700 ± 0.061	0.324 ± 0.025
Zn²⁺ plus heparin	0.002 ± 0.005	0.149 ± 0.003

Additives	HK binding to
Additives	VN multimeric	VN native
None	0.024 ± 0.030	0.002 ± 0.001
Heparin	0.005 ± 0.013	0.001 ± 0.001
Zn²⁺	0.025 ± 0.012	0.026 ± 0.033
Zn²⁺ plus heparin	0.001 ± 0.016	0.003 ± 0.007

Additives	HKa binding to
Additives	VN multimeric	VN native
None	0.023 ± 0.011	0.041 ± 0.021
Heparin	0.001 ± 0.030	0.008 ± 0.013
Zn²⁺	0.700 ± 0.061	0.324 ± 0.025
Zn²⁺ plus heparin	0.002 ± 0.005	0.149 ± 0.003

The binding of 2 μg/mL HK or HKa to immobilized multimeric or native VN (each 5 μg/mL) is shown. Binding was studied in the absence or presence of Zn²⁺ (50 μmol/L) and heparin (10 μg/mL). Binding of HK to BSA in the absence or presence of Zn²⁺was 0.074 ± 0.001 and 0.182 ± 0.031, respectively. Binding of HKa to BSA in the absence or presence of Zn²⁺was 0.106 ± 0.026 and 0.194 ± 0.031, respectively. This nonspecific binding was subtracted to estimate specific binding. Binding is expressed as absorbance at 405 nm. Data are mean ± SEM (n = 3) of a typical experiment, and similar results were obtained in 3 separate experiments.

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