Table 2. Biochemical characteristics of... | American Society of Hematology

Table 2.

Biochemical characteristics of the patients with CGD

Patient	NBT % positive cells^*	$O_{2}^{-}$ DCF/DHR^†	$O_{2}^{-}$ nmol/min/10⁷ cells^‡	Flavocytochrome b₅₅₈
Patient	NBT % positive cells^*	$O_{2}^{-}$ DCF/DHR^†	$O_{2}^{-}$ nmol/min/10⁷ cells^‡	Heme content pmol/10⁷ cells^2-153	Protein content/ flow cytometry
Normal range (n > 100)	>90	100%	122 ± 26	74 ± 13	+
1 (male)	nd	0%		nd	negative
2 (male)	0		0	0	negative
3 (female)	nd		0	nd	negative
4 (female)	0	0%		nd	negative
5 (male)	0		0.2	0	negative
6 (female)	0	5.0%		<5	negative
7 and 7a (female)	0		0	0	negative
8 (female)	0		nd	nd	negative
9 (female)	0	0	0		negative

Patient	NBT % positive cells^*	$O_{2}^{-}$ DCF/DHR^†	$O_{2}^{-}$ nmol/min/10⁷ cells^‡	Flavocytochrome b₅₅₈
Patient	NBT % positive cells^*	$O_{2}^{-}$ DCF/DHR^†	$O_{2}^{-}$ nmol/min/10⁷ cells^‡	Heme content pmol/10⁷ cells^2-153	Protein content/ flow cytometry
Normal range (n > 100)	>90	100%	122 ± 26	74 ± 13	+
1 (male)	nd	0%		nd	negative
2 (male)	0		0	0	negative
3 (female)	nd		0	nd	negative
4 (female)	0	0%		nd	negative
5 (male)	0		0.2	0	negative
6 (female)	0	5.0%		<5	negative
7 and 7a (female)	0		0	0	negative
8 (female)	0		nd	nd	negative
9 (female)	0	0	0		negative

CGD = chronic granulomatous disease; NBT = nitroblue tetrazolium; DCF = dichlorofluorescein; DHR = dihydrorhodamine; nd = not done.

Using the Nitroblue Tetrazolium slide test.25

By flow cytometry using DHR or DCF. Values represent % fluorescence compared with PMA-stimulated normal cells.

Using the superoxide dismutase-inhibitable rate of cytochrome c reduction.25

Determined spectrophotometrically.26