Flow cytometric analysis of immature and LPS-matured populations of MoDCs
| Cell surface marker . | Immature DCs . | LPS-matured DCs . | ||||
|---|---|---|---|---|---|---|
| % positive . | MCF . | % positive × MCF . | % positive . | MCF . | % positive × MCF . | |
| CD83 (n = 7) | 34.8 ± 10.8 | 0.63 ± 0.26 | 29.6 ± 21.3 | 76.1 ± 8.7 | 1.73 ± 0.59 | 150.2 ± 58.5 |
| CD80 (n = 5) | 26.9 ± 10.7 | 1.12 ± 0.54 | 25.3 ± 15.6 | 60.3 ± 16.2 | 2.23 ± 0.70 | 161.1 ± 58.7 |
| CD86 (n = 5) | 11.4 ± 4.2 | 0.94 ± 0.46 | 9.6 ± 4.5 | 66.1 ± 8.5 | 3.25 ± 1.10 | 220.8 ± 83.6 |
| CD40 (n = 4) | 90.9 ± 9.5 | 8.44 ± 3.35 | 768.4 ± 307.6 | 90.7 ± 9.5 | 20.03 ± 8.66 | 1784.9 ± 751 |
| HLA-DR (n = 5) | 96.4 ± 1.1 | 47.56 ± 18.79 | 4625.9 ± 1868 | 95.9 ± 1.1 | 159.5 ± 54.13 | 15 313.5 ± 5176 |
| CD1a (n = 5) | 71.9 ± 9.9 | 11.70 ± 7.29 | 1055.1 ± 695.2 | 70.9 ± 10.6 | 9.66 ± 5.89 | 858.2 ± 561.9 |
| Cell surface marker . | Immature DCs . | LPS-matured DCs . | ||||
|---|---|---|---|---|---|---|
| % positive . | MCF . | % positive × MCF . | % positive . | MCF . | % positive × MCF . | |
| CD83 (n = 7) | 34.8 ± 10.8 | 0.63 ± 0.26 | 29.6 ± 21.3 | 76.1 ± 8.7 | 1.73 ± 0.59 | 150.2 ± 58.5 |
| CD80 (n = 5) | 26.9 ± 10.7 | 1.12 ± 0.54 | 25.3 ± 15.6 | 60.3 ± 16.2 | 2.23 ± 0.70 | 161.1 ± 58.7 |
| CD86 (n = 5) | 11.4 ± 4.2 | 0.94 ± 0.46 | 9.6 ± 4.5 | 66.1 ± 8.5 | 3.25 ± 1.10 | 220.8 ± 83.6 |
| CD40 (n = 4) | 90.9 ± 9.5 | 8.44 ± 3.35 | 768.4 ± 307.6 | 90.7 ± 9.5 | 20.03 ± 8.66 | 1784.9 ± 751 |
| HLA-DR (n = 5) | 96.4 ± 1.1 | 47.56 ± 18.79 | 4625.9 ± 1868 | 95.9 ± 1.1 | 159.5 ± 54.13 | 15 313.5 ± 5176 |
| CD1a (n = 5) | 71.9 ± 9.9 | 11.70 ± 7.29 | 1055.1 ± 695.2 | 70.9 ± 10.6 | 9.66 ± 5.89 | 858.2 ± 561.9 |
Peripheral blood monocytes were cultured with GM-CSF and IL-4 (both at 100 ng/mL) for 7 days. The cells were then divided into 2 aliquots. To one half LPS (100 ng/mL) was added, and to the other half no addition was made. The cells were then cultured for a further 48 hours before being analyzed by flow cytometry. The percentage expressing various cell surface antigens (±SEM) together with the mean cell fluorescence (MCF) of the whole population of cells (±SEM) under scrutiny are shown together with the product of these 2 variables.