Classification of hematopoietic progenitors recovered from Rab-9 cocultures
| . | CFC per culture* . | |||
|---|---|---|---|---|
| HPP-CFC . | CFU-GM . | CFU-G . | CFU-M . | |
| Day 8 of coculture | ||||
| Rab-9/LXSN | 20 ± 8 | 11 ± 5 | 0 ± 0 | 0 ± 0 |
| Rab-9/LMJSN | 615 ± 23 | 295 ± 18 | 64 ± 7 | 27 ± 14 |
| Day 28 of coculture | ||||
| Rab-9/LXSN | 40 ± 18 | 51 ± 17 | 4 ± 4 | 7 ± 6 |
| Rab-9/LMJSN | 1504 ± 446 | 1608 ± 473 | 400 ± 246 | 304 ± 73 |
| . | CFC per culture* . | |||
|---|---|---|---|---|
| HPP-CFC . | CFU-GM . | CFU-G . | CFU-M . | |
| Day 8 of coculture | ||||
| Rab-9/LXSN | 20 ± 8 | 11 ± 5 | 0 ± 0 | 0 ± 0 |
| Rab-9/LMJSN | 615 ± 23 | 295 ± 18 | 64 ± 7 | 27 ± 14 |
| Day 28 of coculture | ||||
| Rab-9/LXSN | 40 ± 18 | 51 ± 17 | 4 ± 4 | 7 ± 6 |
| Rab-9/LMJSN | 1504 ± 446 | 1608 ± 473 | 400 ± 246 | 304 ± 73 |
Experimental conditions were identical to those described in the legend to Figure 7. Lin− c-kit+ cells (3000 per well) were used to initiate the cocultures. Colony assays were performed in methylcellulose supplemented with SCF, IL-3, and GM-CSF. Colonies were counted on day 8 of colony assays. Numbers shown have not been corrected for subculture ratios, but all cultures were handled in the same way (eg, same feeding frequencies and subculture ratios) in parallel.
Mean ± SD; n = 3.