Table 1.

Classification of hematopoietic progenitors recovered from Rab-9 cocultures

CFC per culture*
HPP-CFCCFU-GM CFU-G CFU-M
Day 8 of coculture  
 Rab-9/LXSN 20 ± 8  11 ± 5  0 ± 0  0 ± 0 
 Rab-9/LMJSN  615 ± 23  295 ± 18  64 ± 7 27 ± 14  
Day 28 of coculture  
 Rab-9/LXSN 40 ± 18  51 ± 17  4 ± 4  7 ± 6 
 Rab-9/LMJSN  1504 ± 446  1608 ± 473 400 ± 246  304 ± 73 
CFC per culture*
HPP-CFCCFU-GM CFU-G CFU-M
Day 8 of coculture  
 Rab-9/LXSN 20 ± 8  11 ± 5  0 ± 0  0 ± 0 
 Rab-9/LMJSN  615 ± 23  295 ± 18  64 ± 7 27 ± 14  
Day 28 of coculture  
 Rab-9/LXSN 40 ± 18  51 ± 17  4 ± 4  7 ± 6 
 Rab-9/LMJSN  1504 ± 446  1608 ± 473 400 ± 246  304 ± 73 

Experimental conditions were identical to those described in the legend to Figure 7. Lin c-kit+ cells (3000 per well) were used to initiate the cocultures. Colony assays were performed in methylcellulose supplemented with SCF, IL-3, and GM-CSF. Colonies were counted on day 8 of colony assays. Numbers shown have not been corrected for subculture ratios, but all cultures were handled in the same way (eg, same feeding frequencies and subculture ratios) in parallel.

*

Mean ± SD; n = 3.