Induction of C5a-inhibitor activity in late-passage fibroblast cultures by IL-1β
| Culture . | Passage . | Number of cultures . | Number of experiments . | C5a inhibition (%) . | |
|---|---|---|---|---|---|
| −IL-1β . | +IL-1β . | ||||
| Peritoneal, normal | 5-7 | 6 | 7 | 9.6 ± 3.4 | 23.2 ± 2.63-150 |
| Skin, normal | 7-12 | 2 | 4 | 3.6 ± 1.8 | 8.8 ± 3.9 |
| Peritoneal, FMF | 6 | 1 | 2 | 0.2 ± 0.2 | −3.7 ± 0.9 |
| Culture . | Passage . | Number of cultures . | Number of experiments . | C5a inhibition (%) . | |
|---|---|---|---|---|---|
| −IL-1β . | +IL-1β . | ||||
| Peritoneal, normal | 5-7 | 6 | 7 | 9.6 ± 3.4 | 23.2 ± 2.63-150 |
| Skin, normal | 7-12 | 2 | 4 | 3.6 ± 1.8 | 8.8 ± 3.9 |
| Peritoneal, FMF | 6 | 1 | 2 | 0.2 ± 0.2 | −3.7 ± 0.9 |
FMF indicates familial Mediterranean fever. C5a inhibition was measured as described (see footnote to Table 1) after culture induction with either IL-1β (10 ng/mL for 24 hours) or medium, as described in “Materials and methods.” A490 readings for control myeloperoxidase release were 0.29 to 0.75.
P < .01 (versus noninduced cultures).