Induction of C5a-inhibitor activity in various fibroblast cultures by phorbol myristate acetate (PMA)
| Culture . | Passages . | Number of cultures . | Number of experiments . | C5a inhibition (%) . | |
|---|---|---|---|---|---|
| −PMA . | +PMA . | ||||
| Peritoneal, normal | 3-5 | 4 | 4 | 46.5 ± 6.6 | 40.3 ± 5.1 |
| Peritoneal, normal | 5-6 | 4 | 1 | 14.1 ± 4.2 | 27.0 ± 7.1* |
| Peritoneal, normal | 6-12 | 9 | 15 | 1.5 ± 2.8 | 21.5 ± 2.7† |
| Peritoneal, FMF | 3 | 3 | 8 | −9.0 ± 0.9 | 3.0 ± 2.9 |
| Skin, normal | 3-5 | 3 | 3 | −3.3 ± 6.2 | 18.1 ± 6.6* |
| Skin, FMF | 3-5 | 2 | 6 | 8.4 ± 1.5 | 7.2 ± 0.7 |
| Culture . | Passages . | Number of cultures . | Number of experiments . | C5a inhibition (%) . | |
|---|---|---|---|---|---|
| −PMA . | +PMA . | ||||
| Peritoneal, normal | 3-5 | 4 | 4 | 46.5 ± 6.6 | 40.3 ± 5.1 |
| Peritoneal, normal | 5-6 | 4 | 1 | 14.1 ± 4.2 | 27.0 ± 7.1* |
| Peritoneal, normal | 6-12 | 9 | 15 | 1.5 ± 2.8 | 21.5 ± 2.7† |
| Peritoneal, FMF | 3 | 3 | 8 | −9.0 ± 0.9 | 3.0 ± 2.9 |
| Skin, normal | 3-5 | 3 | 3 | −3.3 ± 6.2 | 18.1 ± 6.6* |
| Skin, FMF | 3-5 | 2 | 6 | 8.4 ± 1.5 | 7.2 ± 0.7 |
FMF indicates familial Mediterranean fever. C5a inhibition was measured as described (see footnote to Table 1) after culture induction with either PMA (100 nmol/L for 1 hour) or medium as described in “Materials and methods.” A490 readings for control myeloperoxidase release were 0.21 to 0.64. For each FMF culture with or without PMA, a normal control culture at the same passage was assayed.
P < .05 (versus noninduced cultures).
P < .001 (versus noninduced cultures).