Interactions of GMR- and IL-3- with GRAP in a yeast 2-hybrid system
| DNA-binding hybrid . | Activation partner . | Color* . |
|---|---|---|
| LexA-GMR-α | GAD | White |
| LexA-GMR-α | GAD-GRAP | Blue |
| LexA | GAD-GRAP | White |
| LexA-Lamin | GAD-GRAP | White |
| LexA-IL-3-α | GAD-GRAP | White |
| DNA-binding hybrid . | Activation partner . | Color* . |
|---|---|---|
| LexA-GMR-α | GAD | White |
| LexA-GMR-α | GAD-GRAP | Blue |
| LexA | GAD-GRAP | White |
| LexA-Lamin | GAD-GRAP | White |
| LexA-IL-3-α | GAD-GRAP | White |
Four transformants were patched on selective SC-2% glucose plates and grown for 1 day, and the color of the transformants was determined by filter assay.