B-lineage cells in BM samples of XLA patients
| Patient no. . | B-lineage cells in BM lymphoid cells, total % . | Percentage of cell types within B-lineage cells . | ||||||
|---|---|---|---|---|---|---|---|---|
| μ−SL++ (pro-B cells), % . | μlowSL++ (pre-B1a cells), % . | μlowSL+ (pre-B1b cells), % . | μlowSL− (pre-B2 cells), % . | μhighSL− (B cells), % . | ||||
| P1 | 26.2 | 88.7 | 7.2 | 3.0 | 0.2 | 0.8 | ||
| P2 | 21.0 | 62.3 | 21.2 | 7.2 | 6.7 | 2.3 | ||
| P3 | 27.8 | 72.8 | 20.5 | 2.8 | 1.1 | 2.7 | ||
| P4 | 41.5 | 77.8 | 15.4 | 4.1 | 1.8 | 0.1 | ||
| P5 | 9.8 | 56.2 | 20.0 | 9.0 | 7.7 | 4.9 | ||
| P6 | 18.5 | 76.8 | 14.8 | 3.9 | 3.1 | 1.0 | ||
| P7 | 29.1 | 66.2 | 17.5 | 8.2 | 5.7 | 1.8 | ||
| P8 | 19.3 | 71.2 | 15.0 | 5.0 | 6.6 | 1.1 | ||
| P9 | 18.9 | 67.3 | 20.2 | 6.7 | 2.7 | 1.7 | ||
| P10 | 14.3 | 73.2 | 10.0 | 5.2 | 5.1 | 6.4 | ||
| P11 | 11.0 | 82.6 | 11.3 | 3.6 | 1.7 | 0.2 | ||
| P12 | 10.4 | 91.6 | 5.0 | 2.0 | 0.1 | 0.7 | ||
| P13 | 8.9 | 80.0 | 10.6 | 2.5 | 0.9 | 0.7 | ||
| Patient no. . | B-lineage cells in BM lymphoid cells, total % . | Percentage of cell types within B-lineage cells . | ||||||
|---|---|---|---|---|---|---|---|---|
| μ−SL++ (pro-B cells), % . | μlowSL++ (pre-B1a cells), % . | μlowSL+ (pre-B1b cells), % . | μlowSL− (pre-B2 cells), % . | μhighSL− (B cells), % . | ||||
| P1 | 26.2 | 88.7 | 7.2 | 3.0 | 0.2 | 0.8 | ||
| P2 | 21.0 | 62.3 | 21.2 | 7.2 | 6.7 | 2.3 | ||
| P3 | 27.8 | 72.8 | 20.5 | 2.8 | 1.1 | 2.7 | ||
| P4 | 41.5 | 77.8 | 15.4 | 4.1 | 1.8 | 0.1 | ||
| P5 | 9.8 | 56.2 | 20.0 | 9.0 | 7.7 | 4.9 | ||
| P6 | 18.5 | 76.8 | 14.8 | 3.9 | 3.1 | 1.0 | ||
| P7 | 29.1 | 66.2 | 17.5 | 8.2 | 5.7 | 1.8 | ||
| P8 | 19.3 | 71.2 | 15.0 | 5.0 | 6.6 | 1.1 | ||
| P9 | 18.9 | 67.3 | 20.2 | 6.7 | 2.7 | 1.7 | ||
| P10 | 14.3 | 73.2 | 10.0 | 5.2 | 5.1 | 6.4 | ||
| P11 | 11.0 | 82.6 | 11.3 | 3.6 | 1.7 | 0.2 | ||
| P12 | 10.4 | 91.6 | 5.0 | 2.0 | 0.1 | 0.7 | ||
| P13 | 8.9 | 80.0 | 10.6 | 2.5 | 0.9 | 0.7 | ||
Different cell types within the B-lineage cells in BM samples were analyzed by 3-color fluorescence in flow cytometry, as shown in the legend of Figure 3. B-lineage cells were discriminated from other lymphoid cells by a combined assessment of CD19 and VpreB expression.