A comparison of XY FISH and BCR-ABL RT-PCR in 11 CML patients after sex-mismatched BMT
| Patient no. . | Months after BMT . | % host by XY FISH (no. of cells) . | % BCR-ABL positive by FISH (no. of cells) . | RT-PCR BCR-ABL/ABL ratio, % . |
|---|---|---|---|---|
| 1 | 15 | 0.3 (1000) | 1 (200) | 1.0 |
| 2 | 28 | 0.2 (1000) | ND | 0.03 |
| 3 | 19 | 0.2 (515) | ND | —* |
| 4 | 7.5 | 0 (500) | ND | —* |
| 5 | 33 | 0 (500) | ND | —* |
| 6 | 9 | 0 (500) | 0.5 (200) | —* |
| 7 | 18 | 0 (500) | 0 (200) | —* |
| 8 | 182 | 0 (500) | ND | —* |
| 9 | 2 | 0 (500) | ND | —* |
| 10 | 7 | 0 (1000) | ND | —* |
| 11 | 72 | 0 (503) | ND | —* |
| Patient no. . | Months after BMT . | % host by XY FISH (no. of cells) . | % BCR-ABL positive by FISH (no. of cells) . | RT-PCR BCR-ABL/ABL ratio, % . |
|---|---|---|---|---|
| 1 | 15 | 0.3 (1000) | 1 (200) | 1.0 |
| 2 | 28 | 0.2 (1000) | ND | 0.03 |
| 3 | 19 | 0.2 (515) | ND | —* |
| 4 | 7.5 | 0 (500) | ND | —* |
| 5 | 33 | 0 (500) | ND | —* |
| 6 | 9 | 0 (500) | 0.5 (200) | —* |
| 7 | 18 | 0 (500) | 0 (200) | —* |
| 8 | 182 | 0 (500) | ND | —* |
| 9 | 2 | 0 (500) | ND | —* |
| 10 | 7 | 0 (1000) | ND | —* |
| 11 | 72 | 0 (503) | ND | —* |
Negative by nested RT-PCR, not quantified.