Metastasis of MDAY-D2 S1 and dominant-negative G11 transfectants
| Location of metastases . | Transfectants . | |||||||
|---|---|---|---|---|---|---|---|---|
| MDAY-D2 . | MDAY-D2 S1 5.3 . | MDAY-D2 Gq-DN . | MDAY-D2 S1 + Gq-DN . | |||||
| Subjects . | Tumor extent . | Subjects . | Tumor extent . | Subjects . | Tumor extent . | Subjects . | Tumor extent . | |
| Liver | 10/10 | ++++ | 0/10 | − | 6/10 | ++ | 0/10 | − |
| Spleen | 10/10 | ++++ | 10/10 | +/− | 5/10 | ++ | 0/10 | − |
| Bone marrow | 10/10 | ++++ | 10/10 | ++++ | 7/10 | +++ | 1/10 | +++ |
| Location of metastases . | Transfectants . | |||||||
|---|---|---|---|---|---|---|---|---|
| MDAY-D2 . | MDAY-D2 S1 5.3 . | MDAY-D2 Gq-DN . | MDAY-D2 S1 + Gq-DN . | |||||
| Subjects . | Tumor extent . | Subjects . | Tumor extent . | Subjects . | Tumor extent . | Subjects . | Tumor extent . | |
| Liver | 10/10 | ++++ | 0/10 | − | 6/10 | ++ | 0/10 | − |
| Spleen | 10/10 | ++++ | 10/10 | +/− | 5/10 | ++ | 0/10 | − |
| Bone marrow | 10/10 | ++++ | 10/10 | ++++ | 7/10 | +++ | 1/10 | +++ |
Cells (2 × 104) were injected intravenously, and the mice were killed at day 15. The number of subjects indicated is given as the number of mice that had metastases per the number of mice injected for each indicated organ. The extent of metastases formation: +/− indicates a few isolated tumor cells; +, some foci; ++, some foci and some diffusely spread tumor cells; +++, more foci and more diffusely spread tumor cells; ++++, many foci and many diffusely spread tumor cells. Data were obtained by macroscopic and microscopic analyses.