Grb4 and Bcr-Abl interaction in yeast
| Constructs . | −TL* . | −THL† . |
|---|---|---|
| F16/Grb4 + WT Bcr-Abl | + | + |
| F16/Grb4 + KD Bcr-Abl | + | − |
| F20/Grb4 + WT Bcr-Abl | + | + |
| F20/Grb4 + KD Bcr-Abl | + | + |
| F16/Grb4 + lamin (control) | + | − |
| F20/Grb4 + lamin (control) | + | − |
| Constructs . | −TL* . | −THL† . |
|---|---|---|
| F16/Grb4 + WT Bcr-Abl | + | + |
| F16/Grb4 + KD Bcr-Abl | + | − |
| F20/Grb4 + WT Bcr-Abl | + | + |
| F20/Grb4 + KD Bcr-Abl | + | + |
| F16/Grb4 + lamin (control) | + | − |
| F20/Grb4 + lamin (control) | + | − |
Interaction of the 2 clones Grb4 F16 and F20 with WT Bcr-Abl, KD Bcr-Abl, or (as a control) lamin in the yeast 2-hybrid screen.
Growth under tryptophan and leucine selection and reflects the transfection efficiency.
Growth under additional histidine selection and reflects the specificity of interaction.