Table 4. Apoptosis in 10 healthy donor... | American Society of Hematology

Table 4.

Apoptosis in 10 healthy donor and 10 HIV-1 patient peripheral blood mononuclear cells stimulated with anti-CD3 antibody/phorbol myristate acetate and cultured with 100 nmol/L indinavir or saquinavir

Duration of culture	Percentage apoptosis (%)^4-150
Duration of culture	Healthy donor PBMCs	HIV-1 patient PBMCs
Day 0
Freshly isolated cells	1.6 ± 1.2	4.6 ± 3.2
Day 7
Medium alone	5.4 ± 3.8	10.3 ± 4.7
Medium + indinavir	4.9 ± 3.5	10.8 ± 4.4
Medium + saquinavir	5.3 ± 3.7	11.5 ± 4.6
Day 14
Medium alone	3.7 ± 2.5	17.9 ± 7.5
Medium + indinavir	4.1 ± 2.8	11.2 ± 4.8^4-151
Medium + saquinavir	3.8 ± 2.2	9.7 ± 4.9^4-151

Duration of culture	Percentage apoptosis (%)^4-150
Duration of culture	Healthy donor PBMCs	HIV-1 patient PBMCs
Day 0
Freshly isolated cells	1.6 ± 1.2	4.6 ± 3.2
Day 7
Medium alone	5.4 ± 3.8	10.3 ± 4.7
Medium + indinavir	4.9 ± 3.5	10.8 ± 4.4
Medium + saquinavir	5.3 ± 3.7	11.5 ± 4.6
Day 14
Medium alone	3.7 ± 2.5	17.9 ± 7.5
Medium + indinavir	4.1 ± 2.8	11.2 ± 4.8^4-151
Medium + saquinavir	3.8 ± 2.2	9.7 ± 4.9^4-151

PBMCs, peripheral blood mononuclear cells.

F4-150

Expressed as the mean ± SD of % apoptotic cells in donor or patient PBMCs.

F4-151

P value (Wilcoxon) < .01 compared with culture medium alone.

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