Table 3.

Hydrolysis of spectrozyme PCa by wild-type and 306-314AAAA-APC

Km mmol/Lkcat s−1kcat/Km M−1 s−1
Wild-type  0.44 ± 0.04 57 ± 4   1.3 × 105 
306-314AAAA 0.50 ± 0.08  49 ± 3 0.98 × 105 
Km mmol/Lkcat s−1kcat/Km M−1 s−1
Wild-type  0.44 ± 0.04 57 ± 4   1.3 × 105 
306-314AAAA 0.50 ± 0.08  49 ± 3 0.98 × 105 
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