Table 1.

Rate constants for APC cleavages of FVa and Q506 FVa

Mutant APC sequence 306-314 FVa k506 M−1 s−1k306 M−1 s−1Q506-FVa k′306 M−1 s−1
Wild-type  REKEAKRNR 2.5 × 108 7.7 × 106 4.6 × 106 
306A  AEKEAKRNR 8.8 × 107 6.7 × 106 3.1 × 106 
307A  RAKEAKRNR 1.5 × 108 8.7 × 106 4.5 × 106 
308A  REAEAKRNR 2.3 × 108 9.3 × 106 4.6 × 106 
309A  REKAAKRNR 2.2 × 108 7.0 × 106 4.7 × 106 
311A  REKEAARNR 6.2 × 107 3.8 × 106 2.9 × 106 
312A  REKEAKANR 6.0 × 107 3.8 × 106 3.1 × 106 
314A  REKEAKRNA 9.0 × 107 4.3 × 106 1.9 × 106 
306/312AA  AEKEAKANR 2.2 × 107 3.9 × 106 2.5 × 106 
306-314AAAA  AEKEAAANA 3.5 × 106 1.2 × 106 9.7 × 105 
Mutant APC sequence 306-314 FVa k506 M−1 s−1k306 M−1 s−1Q506-FVa k′306 M−1 s−1
Wild-type  REKEAKRNR 2.5 × 108 7.7 × 106 4.6 × 106 
306A  AEKEAKRNR 8.8 × 107 6.7 × 106 3.1 × 106 
307A  RAKEAKRNR 1.5 × 108 8.7 × 106 4.5 × 106 
308A  REAEAKRNR 2.3 × 108 9.3 × 106 4.6 × 106 
309A  REKAAKRNR 2.2 × 108 7.0 × 106 4.7 × 106 
311A  REKEAARNR 6.2 × 107 3.8 × 106 2.9 × 106 
312A  REKEAKANR 6.0 × 107 3.8 × 106 3.1 × 106 
314A  REKEAKRNA 9.0 × 107 4.3 × 106 1.9 × 106 
306/312AA  AEKEAKANR 2.2 × 107 3.9 × 106 2.5 × 106 
306-314AAAA  AEKEAAANA 3.5 × 106 1.2 × 106 9.7 × 105 
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