Percentage and phenotype of cells undergoing apoptosis on CD4XL in PBT annexin V labeling
| Addition . | Pretreatment . | |||||
|---|---|---|---|---|---|---|
| Medium . | mIgG/GAM . | Leu3a/GAM . | ||||
| CD4+ T cells . | CD8+ T cells . | CD4+ T cells . | CD8+ T cells . | CD4+ T cells . | CD8+ T cells . | |
| Added after 40 minutes | ||||||
| None | 8.1 ± 1.4 | 8.9 ± 2.1 | 10.2 ± 2.2 | 10.9 ± 2.5 | 14.4 ± 3.2 | 11.1 ± 2.7 |
| Anti-CD3 mAb | 8.6 ± 2.3 | 9.5 ± 2.0 | 11.2 ± 1.3 | 10.7 ± 1.9 | 13.4 ± 3.1 | 13.0 ± 1.9 |
| Anti-CD3/CD28 mAbs | 8.9 ± 1.8 | 10.2 ± 1.4 | 9.8 ± 1.8 | 10.0 ± 1.7 | 12.7 ± 2.4 | 9.5 ± 1.3 |
| Added after 24 hours | ||||||
| None | 9.2 ± 3.2 | 8.8 ± 1.9 | 10.7 ± 3.9 | 11.2 ± 4.0 | 12.1 ± 3.3 | 7.4 ± 1.9 |
| Anti-CD3 mAb | 9.6 ± 4.0 | 10.2 ± 3.6 | 10.4 ± 2.8 | 9.8 ± 2.2 | 32.7 ± 5.9* | 39.5 ± 5.8* |
| Anti-CD3/CD28 mAbs | 8.8 ± 2.3 | 9.7 ± 3.7 | 11.3 ± 3.4 | 11.9 ± 3.7 | 18.3 ± 2.1 | 10.3 ± 2.3 |
| Addition . | Pretreatment . | |||||
|---|---|---|---|---|---|---|
| Medium . | mIgG/GAM . | Leu3a/GAM . | ||||
| CD4+ T cells . | CD8+ T cells . | CD4+ T cells . | CD8+ T cells . | CD4+ T cells . | CD8+ T cells . | |
| Added after 40 minutes | ||||||
| None | 8.1 ± 1.4 | 8.9 ± 2.1 | 10.2 ± 2.2 | 10.9 ± 2.5 | 14.4 ± 3.2 | 11.1 ± 2.7 |
| Anti-CD3 mAb | 8.6 ± 2.3 | 9.5 ± 2.0 | 11.2 ± 1.3 | 10.7 ± 1.9 | 13.4 ± 3.1 | 13.0 ± 1.9 |
| Anti-CD3/CD28 mAbs | 8.9 ± 1.8 | 10.2 ± 1.4 | 9.8 ± 1.8 | 10.0 ± 1.7 | 12.7 ± 2.4 | 9.5 ± 1.3 |
| Added after 24 hours | ||||||
| None | 9.2 ± 3.2 | 8.8 ± 1.9 | 10.7 ± 3.9 | 11.2 ± 4.0 | 12.1 ± 3.3 | 7.4 ± 1.9 |
| Anti-CD3 mAb | 9.6 ± 4.0 | 10.2 ± 3.6 | 10.4 ± 2.8 | 9.8 ± 2.2 | 32.7 ± 5.9* | 39.5 ± 5.8* |
| Anti-CD3/CD28 mAbs | 8.8 ± 2.3 | 9.7 ± 3.7 | 11.3 ± 3.4 | 11.9 ± 3.7 | 18.3 ± 2.1 | 10.3 ± 2.3 |
PBT were analyzed for annexin V positive cells to determine percentage of cells undergoing apoptosis. Phenotypes were determined by surface labeling with anti-CD8 mAb as described. Data represent mean ± SD of 3 experiments.
Abbreviations: CD4XL, CD4 cross-linking; PBT, peripheral blood T cells; mIgG, mouse immunoglobulin G1; GAM, goat anti-mouse; mAb, monoclonal antibody.
P < .05 as determined by paired test compared with medium treatment instead of CD4XL.