Table 2. ET depresses T-cell... | American Society of Hematology

Table 2.

ET depresses T-cell proliferation and IFN-γ production in response to various protein recall antigens

			Control	LPS primed
A	³H incorporation	Can	29.5 ± 11.30	9.0 ± 4.15^*
	[specific cpm × 10³/culture]	TT	34.3 ± 9.55	5.2 ± 2.41^*
		Tbc	33.5 ± 9.73	3.5 ± 1.50^*
B	IFN-γ [U/mL]	Can	17.2 ± 10.80	3.3 ± 1.80^*
		TT	9.5 ± 4.00	1.6 ± 0.20^*
		Tbc	21.1 ± 5.70	2.2 ± 1.00^*

			Control	LPS primed
A	³H incorporation	Can	29.5 ± 11.30	9.0 ± 4.15^*
	[specific cpm × 10³/culture]	TT	34.3 ± 9.55	5.2 ± 2.41^*
		Tbc	33.5 ± 9.73	3.5 ± 1.50^*
B	IFN-γ [U/mL]	Can	17.2 ± 10.80	3.3 ± 1.80^*
		TT	9.5 ± 4.00	1.6 ± 0.20^*
		Tbc	21.1 ± 5.70	2.2 ± 1.00^*

PBMCs were cultured in the presence or absence of 2 ng/mL LPS. After 24 hours, cells were washed and recultured in the presence of either 2.5 μg/mL candidin (Can), 0.08 IU/mL tetanus toxoid (TT), 5 PPD-S/mL tuberculin (Tbc), or without stimulation. (A) Cellular [5′-³H]thymidine incorporation after 3 days of secondary culture is given as specific cpm (Figure 3). (B) IFN-γ concentrations in culture supernatant were assessed by ELISA after 4 days of secondary culture. Mean (±SEM) data from 6 independent experiments are given. Statistical analysis was performed by Wilcoxon matched-pairs signed-rank test (*P < .05 versus untreated cultures).

IFN indicates interferon; Can, candidin; TT, tetanus toxoid; Tbc, tuberculin.

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