Table 1. Expression of different MHC... | American Society of Hematology

Table 1.

Expression of different MHC class II species on monocytes is down-regulated during ET; a comparison with monocyte TNF- production capacity

	Control	LPS primed
HLA-DR [MFI]	2427.4 ± 514.85	267.8 ± 86.21^*
HLA-DQ [MFI]	529.1 ± 164.15	66.6 ± 42.24^*
HLA-DP [MFI]	1731.5 ± 620.01	136.1 ± 49.67^*
TNFα [pg/mL]	5113.8 ± 2510.00	50.8 ± 16.57^*

	Control	LPS primed
HLA-DR [MFI]	2427.4 ± 514.85	267.8 ± 86.21^*
HLA-DQ [MFI]	529.1 ± 164.15	66.6 ± 42.24^*
HLA-DP [MFI]	1731.5 ± 620.01	136.1 ± 49.67^*
TNFα [pg/mL]	5113.8 ± 2510.00	50.8 ± 16.57^*

PBMCs were cultured with (2 ng/mL) or without LPS for 24 hours and subsequently washed. Monocyte HLA-DR, HLA-DP, and HLA-DQ expression was analyzed by flow cytometry at 36 hours. In parallel, cells were restimulated at 36 hours with 100 ng/mL LPS and 6 hours later TNF-α concentrations in culture supernatant were assessed by ELISA. Mean (±SEM) data from 5 independent experiments are presented. Statistical analysis was performed by Wilcoxon matched-pairs signed-rank test (*P < .05 versus untreated cultures).

MFI indicates mean fluorescence intensity; TNF, tumor necrosis factor.

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