Effect of depletion of Ly-49A+ and Ly-49G2+ NK cells in different F1 mice on the rejection of BMC allografts
| BMC donor . | H2 . | BMC recipient . | H2 . | Recipient treatment . | Total CFU-c/spleen (×106) . |
|---|---|---|---|---|---|
| Experiment A | |||||
| C57BL/10 | b | B10.BR × B10.D2 | k × d | NRS | 0 ± 0 |
| 4D11 (anti-Ly-49G2) | 55 ± 30 | ||||
| YE-148 (anti-Ly-49A) | 2 ± 3 | ||||
| 4D11 + YE1-48 | 2946 ± 16654-150 | ||||
| PK136 (anti-NK1.1) | 2268 ± 549 | ||||
| Experiment B | |||||
| C57BL/10 | b | B10.D2 × B10 | b × d | NRS | 0 ± 0 |
| 4D11 (anti-Ly-49G2) | 97 ± 36 | ||||
| YE1-48 (anti-Ly-49A) | 41 ± 22 | ||||
| 4D11 + YE1-48 | 2941 ± 6584-151 | ||||
| PK136 (anti-NK1.1) | 1404 ± 406 | ||||
| Experiment C | |||||
| C57BL/6 | b | B10.BR × B10 | k × b | NRS | 10 ± 13 |
| 4D11 (anti-Ly-49G2) | 8 ± 7 | ||||
| YE1-48 (anti-Ly-49A) | 105 ± 41 | ||||
| 4D11 + YE1-48 | 267 ± 94‡ | ||||
| PK136 (anti-NK1.1) | 225 ± 37 |
| BMC donor . | H2 . | BMC recipient . | H2 . | Recipient treatment . | Total CFU-c/spleen (×106) . |
|---|---|---|---|---|---|
| Experiment A | |||||
| C57BL/10 | b | B10.BR × B10.D2 | k × d | NRS | 0 ± 0 |
| 4D11 (anti-Ly-49G2) | 55 ± 30 | ||||
| YE-148 (anti-Ly-49A) | 2 ± 3 | ||||
| 4D11 + YE1-48 | 2946 ± 16654-150 | ||||
| PK136 (anti-NK1.1) | 2268 ± 549 | ||||
| Experiment B | |||||
| C57BL/10 | b | B10.D2 × B10 | b × d | NRS | 0 ± 0 |
| 4D11 (anti-Ly-49G2) | 97 ± 36 | ||||
| YE1-48 (anti-Ly-49A) | 41 ± 22 | ||||
| 4D11 + YE1-48 | 2941 ± 6584-151 | ||||
| PK136 (anti-NK1.1) | 1404 ± 406 | ||||
| Experiment C | |||||
| C57BL/6 | b | B10.BR × B10 | k × b | NRS | 10 ± 13 |
| 4D11 (anti-Ly-49G2) | 8 ± 7 | ||||
| YE1-48 (anti-Ly-49A) | 105 ± 41 | ||||
| 4D11 + YE1-48 | 267 ± 94‡ | ||||
| PK136 (anti-NK1.1) | 225 ± 37 |
Mice were given various mAb treatments or NRS 2 days before irradiation. Irradiated mice (4 mice/group) received 2 × 106 (experiments A and B) or 0.5 × 106(experiment C) allogeneic BMC. Eight days later a soft-agar colony assay was grown to assess hematopoietic progenitor content.
Values significantly greater (P < .04) than B10.BR × B10.D2 in mice receiving 4D11 or YE1-48 alone.
Values significantly greater (P < .003) than B10.D2 × B10 in mice receiving 4D11 or YE1-48 alone.
Values significantly greater (P < .03) than B10.BR × B10 in mice receiving 4D11 or YE1-48 alone.