Effect of depletion of Ly-49A+ and Ly-49G2+ NK cells in B10.D2 (H2d) mice on the rejection of BMC allografts
BMC donor . | H2 . | BMC recipient . | H2 . | Recipient treatment . | Total CFU-c/spleen (×106) . |
---|---|---|---|---|---|
Experiment A | |||||
C57BL/10 | b | B10.D2 | d | NRS | 0 ± 0 |
4D11 (anti-Ly-49G2) | 0 ± 0 | ||||
YE1-48 (anti-Ly-49A) | 0 ± 0 | ||||
PK136 (anti-NK1.1) | 267 ± 423-150 | ||||
Experiment B | |||||
C57BL/10 | b | B10.D2 | d | NRS | 0 ± 0 |
4D11 (anti-Ly-49G2) | 9 ± 18 | ||||
YE1-48 (anti-Ly-49A) | 0 ± 0 | ||||
4D11 + YE1-48 | 3244 ± 3493-151 | ||||
PK136 (anti-NK1.1) | 885 ± 438 |
BMC donor . | H2 . | BMC recipient . | H2 . | Recipient treatment . | Total CFU-c/spleen (×106) . |
---|---|---|---|---|---|
Experiment A | |||||
C57BL/10 | b | B10.D2 | d | NRS | 0 ± 0 |
4D11 (anti-Ly-49G2) | 0 ± 0 | ||||
YE1-48 (anti-Ly-49A) | 0 ± 0 | ||||
PK136 (anti-NK1.1) | 267 ± 423-150 | ||||
Experiment B | |||||
C57BL/10 | b | B10.D2 | d | NRS | 0 ± 0 |
4D11 (anti-Ly-49G2) | 9 ± 18 | ||||
YE1-48 (anti-Ly-49A) | 0 ± 0 | ||||
4D11 + YE1-48 | 3244 ± 3493-151 | ||||
PK136 (anti-NK1.1) | 885 ± 438 |
Mice were given various mAb treatments or NRS 2 days before irradiation. Irradiated mice (4 mice/group) received 1 × 106 (experiment A) or 2 × 106 (experiment B) allogeneic BMC. Eight days later a soft-agar colony assay was grown to assess hematopoietic progenitor content.
Values significantly greater (P < .008) than in mice receiving NRS in experiment A.
Values significantly greater (P < .003) than in mice receiving mAb 4D11 or YE1-48 alone in experiment B.