Table 3.

Effect of depletion of Ly-49A+ and Ly-49G2+ NK cells in B10.D2 (H2d) mice on the rejection of BMC allografts

BMC donor H2 BMC recipient H2 Recipient treatment Total CFU-c/spleen (×106)
Experiment A  
 C57BL/10  b  B10.D2  d  NRS 0 ± 0  
    4D11 (anti-Ly-49G2)  0 ± 0 
    YE1-48 (anti-Ly-49A)  0 ± 0 
    PK136 (anti-NK1.1)  267 ± 423-150 
Experiment B  
 C57BL/10  b  B10.D2  d  NRS 0 ± 0  
    4D11 (anti-Ly-49G2)  9 ± 18 
    YE1-48 (anti-Ly-49A)  0 ± 0 
    4D11 + YE1-48  3244 ± 3493-151 
    PK136 (anti-NK1.1)  885 ± 438 
BMC donor H2 BMC recipient H2 Recipient treatment Total CFU-c/spleen (×106)
Experiment A  
 C57BL/10  b  B10.D2  d  NRS 0 ± 0  
    4D11 (anti-Ly-49G2)  0 ± 0 
    YE1-48 (anti-Ly-49A)  0 ± 0 
    PK136 (anti-NK1.1)  267 ± 423-150 
Experiment B  
 C57BL/10  b  B10.D2  d  NRS 0 ± 0  
    4D11 (anti-Ly-49G2)  9 ± 18 
    YE1-48 (anti-Ly-49A)  0 ± 0 
    4D11 + YE1-48  3244 ± 3493-151 
    PK136 (anti-NK1.1)  885 ± 438 

Mice were given various mAb treatments or NRS 2 days before irradiation. Irradiated mice (4 mice/group) received 1 × 106 (experiment A) or 2 × 106 (experiment B) allogeneic BMC. Eight days later a soft-agar colony assay was grown to assess hematopoietic progenitor content.

F3-150

Values significantly greater (P < .008) than in mice receiving NRS in experiment A.

F3-151

Values significantly greater (P < .003) than in mice receiving mAb 4D11 or YE1-48 alone in experiment B.

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