Effect of depletion of Ly-49A+ and Ly-49G2+ NK cells in B10.BR (H2k) mice on the rejection of H2b allografts
| BMC donor . | H2 . | BMC recipient . | H2 . | Recipient treatment . | Total CFU-c/spleen (×106) . |
|---|---|---|---|---|---|
| Experiment A | |||||
| C57BL/10 | b | B10.BR | k | NRS | 0 ± 0 |
| 4D11 (anti-Ly-49G2) | 56 ± 27 | ||||
| YE1-48 (anti-Ly-49A) | 371 ± 84* | ||||
| PK136 (anti-NK1.1) | 1057 ± 258* | ||||
| Experiment B | |||||
| C57BL/10 | b | B10.BR | k | NRS | 0 ± 0 |
| 4D11 (anti-Ly-49G2) | 123 ± 139 | ||||
| YE1-48 (anti-Ly-49A) | 453 ± 212 | ||||
| 4D11 + YE1-48 | 1622 ± 435† | ||||
| PK136 (anti-NK1.1) | 1732 ± 344† |
| BMC donor . | H2 . | BMC recipient . | H2 . | Recipient treatment . | Total CFU-c/spleen (×106) . |
|---|---|---|---|---|---|
| Experiment A | |||||
| C57BL/10 | b | B10.BR | k | NRS | 0 ± 0 |
| 4D11 (anti-Ly-49G2) | 56 ± 27 | ||||
| YE1-48 (anti-Ly-49A) | 371 ± 84* | ||||
| PK136 (anti-NK1.1) | 1057 ± 258* | ||||
| Experiment B | |||||
| C57BL/10 | b | B10.BR | k | NRS | 0 ± 0 |
| 4D11 (anti-Ly-49G2) | 123 ± 139 | ||||
| YE1-48 (anti-Ly-49A) | 453 ± 212 | ||||
| 4D11 + YE1-48 | 1622 ± 435† | ||||
| PK136 (anti-NK1.1) | 1732 ± 344† |
Mice were given various mAb treatments or NRS 2 days before irradiation. Irradiated mice (4 mice/group) received 1 × 106 allogeneic BMC. Eight days later a soft-agar colony assay was grown to assess hematopoietic progenitor content.
Values significantly greater (P < .003) than in mice receiving NRS and mAb 4D11.
Values significantly greater (P < .005) than in mice receiving mAb 4D11 or YE1-48 in experiment B.