Complement lysis of fresh follicular non-Hodgkin's lymphoma samples
| Condition . | Patient 1 . | Patient 2 . | Patient 3 . |
|---|---|---|---|
| Medium | 0 | 0 | 0 |
| NHS4-150 | 0 | 2.3 (2.3) | 0 |
| R4-151 | 6.1 (6.1) | 3.2 (3.2) | 4.9 (2.5) |
| NHS + R | 59.4 (0.4) | 51.2 (1.3) | 85 (5.1) |
| NHS + R + anti-CD46 | 54.9 (2.9) | 50.6 (2.1) | 71.8 (4.8) |
| NHS + R + anti-CD55 | 89.7 (2.2)‡ | 58.0 (3.9) | 95 (3.2) |
| NHS + R + anti-CD59 | 83.6 (6.5)4-153 | 54.8 (9.4) | 82.7 (2.6) |
| NHS + R + anti-CD55/CD59 | 93 (3)‡ | 55.9 (5.0) | 93.1 (2.5) |
| Condition . | Patient 1 . | Patient 2 . | Patient 3 . |
|---|---|---|---|
| Medium | 0 | 0 | 0 |
| NHS4-150 | 0 | 2.3 (2.3) | 0 |
| R4-151 | 6.1 (6.1) | 3.2 (3.2) | 4.9 (2.5) |
| NHS + R | 59.4 (0.4) | 51.2 (1.3) | 85 (5.1) |
| NHS + R + anti-CD46 | 54.9 (2.9) | 50.6 (2.1) | 71.8 (4.8) |
| NHS + R + anti-CD55 | 89.7 (2.2)‡ | 58.0 (3.9) | 95 (3.2) |
| NHS + R + anti-CD59 | 83.6 (6.5)4-153 | 54.8 (9.4) | 82.7 (2.6) |
| NHS + R + anti-CD55/CD59 | 93 (3)‡ | 55.9 (5.0) | 93.1 (2.5) |
The % lysed cells was determined by acridine orange and trypan blue exclusion, in triplicates, after 3 hours incubation in the indicated conditions. The results are the means and range of 2 separate experiments. The statistical significance was calculated from the blocking antibody treated samples relative to the those incubated with NHS + R only.
NHS = normal human serum; R = Rituximab.
NHS was used at 25%.
5 μg/ml of Rituximab was used.
P < .01 (Student t test).
P < .05.