Rituximab does not induce apoptosis in DHL-4 cells
| Time . | Control . | +Rituximab (10 μg/mL) . | +Staurosporine (1 μmol/L) . | |||
|---|---|---|---|---|---|---|
| Apoptotic . | Necrotic . | Apoptotic . | Necrotic . | Apoptotic . | Necrotic . | |
| 0 | 0 | 1.5 | 0 | 1.5 | 1.0 | 1.5 |
| 4h | ND | ND | 1.3 | 1.6 | 17.2 | 4.2 |
| 24h | 0.3 | 1.0 | 0.3 | 1.8 | 2.4 | 32.9 |
| 48h | 0.1 | 1.8 | 0.2 | 2.1 | 0.6 | 74.6 |
| 72h | 0.1 | 1.9 | 0.2 | 3.6 | ND | ND |
| 96h | 0.1 | 1.9 | 0.2 | 2.6 | ND | ND |
| Time . | Control . | +Rituximab (10 μg/mL) . | +Staurosporine (1 μmol/L) . | |||
|---|---|---|---|---|---|---|
| Apoptotic . | Necrotic . | Apoptotic . | Necrotic . | Apoptotic . | Necrotic . | |
| 0 | 0 | 1.5 | 0 | 1.5 | 1.0 | 1.5 |
| 4h | ND | ND | 1.3 | 1.6 | 17.2 | 4.2 |
| 24h | 0.3 | 1.0 | 0.3 | 1.8 | 2.4 | 32.9 |
| 48h | 0.1 | 1.8 | 0.2 | 2.1 | 0.6 | 74.6 |
| 72h | 0.1 | 1.9 | 0.2 | 3.6 | ND | ND |
| 96h | 0.1 | 1.9 | 0.2 | 2.6 | ND | ND |
ND, Not determined.
Apoptotic cells are Annexin V positive and propidium iodide negative.
Necrotic cells are double positive for Annexin V and propidium iodide.