C albicans infection results in higher frequency of Mac-1+Gr-1lo cells in peripheral blood of knockout mice compared to wild-type mice
| Genotype . | Leukocyte (109/L)4-150 . | Mac-1+Gr-1lo cells (%) . | Mac-1 expression (mean fluorescence intensity) . |
|---|---|---|---|
| Wild-type | 8.4 ± 0.8 | 2.3 ± 0.9 | 264 ± 31 |
| G-CSF−/− | 6.6 ± 0.1 | 6.2 ± 1.34-151 | 436 ± 62‡ |
| G-CSF−/−/GM-CSF−/− | 5.3 ± 0.9 | 8.2 ± 1.64-151 | 486 ± 95‡ |
| G-CSF−/−/IL-6−/− | 6.2 ± 0.4 | 6.9 ± 2.4 | 396 ± 50 |
| Genotype . | Leukocyte (109/L)4-150 . | Mac-1+Gr-1lo cells (%) . | Mac-1 expression (mean fluorescence intensity) . |
|---|---|---|---|
| Wild-type | 8.4 ± 0.8 | 2.3 ± 0.9 | 264 ± 31 |
| G-CSF−/− | 6.6 ± 0.1 | 6.2 ± 1.34-151 | 436 ± 62‡ |
| G-CSF−/−/GM-CSF−/− | 5.3 ± 0.9 | 8.2 ± 1.64-151 | 486 ± 95‡ |
| G-CSF−/−/IL-6−/− | 6.2 ± 0.4 | 6.9 ± 2.4 | 396 ± 50 |
WBCs were incubated with antibodies against myeloid cell markers (Gr-1 and Mac-1) and analyzed on FACScan using CellQuest software. Data are represented as mean ± SD. Ten to 12 mice of each genotype were analyzed.
Total WBC count as determined by Sysmex-K1000.
P < .05 compared to wild-type mice.
P < .05 compared to wild-type mice.