Presence of immature neutrophils in peripheral blood of mice after candida infection
| Genotype . | Leukocytes (109/L) . | Neutrophils (109/L) . | % Cell type (neutrophil lineage) . | |
|---|---|---|---|---|
| Promyelocyte and myelocyte . | Metamyelocyte, band, and polymorph . | |||
| Wild-type | 8.6 ± 2.8 | 2.1 ± 0.1 | 11 ± 5.0 | 87 ± 7.0 |
| G-CSF−/− | 7.9 ± 1.8 | 1.6 ± 1.0 | 31 ± 9.0 | 69 ± 13.0* |
| G-CSF−/−/GM-CSF−/− | 5.1 ± 0.5 | 1.1 ± 0.2 | 46 ± 14.0 | 54 ± 14.0* |
| G-CSF−/−/IL-6−/− | 6.2 ± 0.8 | 1.6 ± 0.3 | 35 ± 9.0 | 65 ± 9.0* |
| Genotype . | Leukocytes (109/L) . | Neutrophils (109/L) . | % Cell type (neutrophil lineage) . | |
|---|---|---|---|---|
| Promyelocyte and myelocyte . | Metamyelocyte, band, and polymorph . | |||
| Wild-type | 8.6 ± 2.8 | 2.1 ± 0.1 | 11 ± 5.0 | 87 ± 7.0 |
| G-CSF−/− | 7.9 ± 1.8 | 1.6 ± 1.0 | 31 ± 9.0 | 69 ± 13.0* |
| G-CSF−/−/GM-CSF−/− | 5.1 ± 0.5 | 1.1 ± 0.2 | 46 ± 14.0 | 54 ± 14.0* |
| G-CSF−/−/IL-6−/− | 6.2 ± 0.8 | 1.6 ± 0.3 | 35 ± 9.0 | 65 ± 9.0* |
Manual 100-count leukocyte differentials and further 100-count differentials of cells of neutrophil lineage were performed on blood smears from age- and sex-matched mice. A total of 6-8 mice of each genotype were analyzed on day 7 after candida infection. Data represent mean ± SD.
P < .05 compared to wild-type mice.