Changes in bone marrow hematopoietic cells in response to candida infection in wild-type and G-CSF–deficient mice
| Genotype . | Days after candida infection* . | Total cellularity† . | Cell type (%) . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Blast . | Nucleated erythrocyte . | Promyelocyte and myelocyte . | Metamyelocyte and polymorph . | Lymphocyte . | Monocyte . | Eosinophil . | |||
| Wild-type | 0 (9) | 3.8 ± 0.3 | 1 ± 0.6 | 27 ± 1 | 13 ± 1 | 23 ± 2 | 25 ± 2 | 9 ± 1 | 2 ± 1 |
| G-CSF−/− | 0 (8) | 3.3 ± 0.2 | 2 ± 1 | 31 ± 2 | 13 ± 2 | 10 ± 2 | 34 ± 3 | 8 ± 1 | 2 ± 1 |
| Wild-type | 3 (9) | 3.5 ± 0.2 | 3 ± 1 | 17 ± 1 | 10 ± 1 | 19 ± 1 | 23 ± 2 | 13 ± 1 | 6 ± 1 |
| G-CSF−/− | 3 (7) | 1.7 ± 0.1‡,1-153 | 11 ± 3 | 20 ± 2 | 18 ± 1‡ | 12 ± 2 | 30 ± 2 | 13 ± 2 | 6 ± 1 |
| Wild-type | 7 (10) | 4.5 ± 0.3‡ | 3 ± 1 | 11 ± 2 | 20 ± 1‡ | 35 ± 3‡ | 13 ± 2 | 15 ± 1‡ | 3 ± 1 |
| G-CSF−/− | 7 (7) | 3.7 ± 0.1 | 4 ± 1 | 19 ± 3 | 27 ± 3‡ | 15 ± 1‡ | 14 ± 2 | 21 ± 4 | 0 ± 1 |
| Genotype . | Days after candida infection* . | Total cellularity† . | Cell type (%) . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Blast . | Nucleated erythrocyte . | Promyelocyte and myelocyte . | Metamyelocyte and polymorph . | Lymphocyte . | Monocyte . | Eosinophil . | |||
| Wild-type | 0 (9) | 3.8 ± 0.3 | 1 ± 0.6 | 27 ± 1 | 13 ± 1 | 23 ± 2 | 25 ± 2 | 9 ± 1 | 2 ± 1 |
| G-CSF−/− | 0 (8) | 3.3 ± 0.2 | 2 ± 1 | 31 ± 2 | 13 ± 2 | 10 ± 2 | 34 ± 3 | 8 ± 1 | 2 ± 1 |
| Wild-type | 3 (9) | 3.5 ± 0.2 | 3 ± 1 | 17 ± 1 | 10 ± 1 | 19 ± 1 | 23 ± 2 | 13 ± 1 | 6 ± 1 |
| G-CSF−/− | 3 (7) | 1.7 ± 0.1‡,1-153 | 11 ± 3 | 20 ± 2 | 18 ± 1‡ | 12 ± 2 | 30 ± 2 | 13 ± 2 | 6 ± 1 |
| Wild-type | 7 (10) | 4.5 ± 0.3‡ | 3 ± 1 | 11 ± 2 | 20 ± 1‡ | 35 ± 3‡ | 13 ± 2 | 15 ± 1‡ | 3 ± 1 |
| G-CSF−/− | 7 (7) | 3.7 ± 0.1 | 4 ± 1 | 19 ± 3 | 27 ± 3‡ | 15 ± 1‡ | 14 ± 2 | 21 ± 4 | 0 ± 1 |
Cell counts and 100-count manual leukocyte differentials were performed on mononuclear cells recovered from femurs of mice. Data are presented as mean ± SD.
Study day 0 is pretreatment; the value in the parentheses is the number of mice studied for that group.
Marrow cellularity is cells ×107/2 femurs.
P < .05 for comparison between mice of the same genotype at baseline and on the study day indicated.
P < .05 for comparison between wild type and G-CSF−/− on the same study day.