Table 1.

Result of analysis of the 5′RACE PCR trapped sequence in the GenBank database

Clone number Gene description 5′RACE flanking fragment size (bp) Overlap length (bp)% Identity in the overlap length GenBank accession number
50  hnRNPL (RBM3)  198  177  100 (h) U28686 
134  NACA  47  47  100  (h) X80909 
18  hnRNPF  86  28  97  (h) AA310292 
30 Unknown  111  62  82  (m) D77412 
165/(192) Unknown  170/33  127  100  (h) AA38369  
11 Unknown  175  50  94  (h) AA77426  
122  Unknown 114  106  97  (h) AA326035 
G  Unknown  79   None  
31  Jumonji  353  202  99.5  (m) D311967  
C  L18a  44  44  100  (r) X14181 
Clone number Gene description 5′RACE flanking fragment size (bp) Overlap length (bp)% Identity in the overlap length GenBank accession number
50  hnRNPL (RBM3)  198  177  100 (h) U28686 
134  NACA  47  47  100  (h) X80909 
18  hnRNPF  86  28  97  (h) AA310292 
30 Unknown  111  62  82  (m) D77412 
165/(192) Unknown  170/33  127  100  (h) AA38369  
11 Unknown  175  50  94  (h) AA77426  
122  Unknown 114  106  97  (h) AA326035 
G  Unknown  79   None  
31  Jumonji  353  202  99.5  (m) D311967  
C  L18a  44  44  100  (r) X14181 

Overlap length is represented as the number of nucleotides present in the 5′RACE PCR sequence matching a GenBank sequence over the total length of the 5′RACE PCR sequence. The sequence of the known gene is present in the 5′RACE PCR sequence for clones 50, 31, 134, and C. For clone 18, the 5′RACE PCR sequence is contained in an EST sequence (AA310292) from which the 3′end overlaps (208/1902) with the 5′end sequence encoding an hnRNPF(L28010). % Identity is calculated with the number of nucleotides with a complete match in the overlapping GenBank sequence. (h) human; (m) mouse; (r) rat.

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