Immunophenotype of leukemic cells generated by unsorted, sorted CD8+ SP, or sorted CD4+CD8+ DP populations following transplantation in nude mice
| Donor animal . | Injected population (%SP; %DP)3-150 . | Transplanted animal3-151 . | Immunophenotype of tumor in transplanted animals . | ||
|---|---|---|---|---|---|
| %CD8+ . | %CD4+CD8+ . | %CD4+ . | |||
| 71-18 | Unsorted (59%; 40%) | Nu18 | 61 | 35 | 0.4 |
| Nu18 | Unsorted (61%; 35%) | Nu18/1 Nu18/2 Nu18/3 | 32 54 44 | 53 26 35 | 1.7 1.5 0.3 |
| Enriched SP (90%; 8%) | Nu18/SP1 Nu18/SP2 Nu18/SP3 Nu18/SP4 | 21 40 61 45 | 71 47 30 42.5 | 2 1.3 0.5 1 | |
| Enriched DP (2%; 97%) | Nu18/DP1 Nu18/DP2 | 41.5 38 | 47 57 | 2 1.5 | |
| 71-24 | Unsorted (23%; 70%) | Nu24 | 83 | 7 | 0.2 |
| Enriched SP (98%; 1%) | Nu/SP24 | 63 | 30 | 0.4 | |
| Nu24 | Unsorted (83%; 7%) | Nu24/1 Nu24/2 | 69 67 | 15 23 | 0.5 0 |
| Enriched SP (90%; 1%) | Nu24/SP1 Nu24/SP2 Nu24/SP3 | 75 80 72 | 15 16 25 | 0.3 0.5 0 | |
| Enriched DP (15%; 79%) | Nu24/DP1 Nu24/DP2 | 67 60 | 30 35 | 0.4 0.5 | |
| 71-43 | Unsorted (63%; 26%) | Nu43/1 Nu43/2 | 43 39 | 56 58 | 0.3 0.6 |
| Enriched SP (96%; 2%) | Nu/SP43-1 | 66 | 33 | 0.2 | |
| Donor animal . | Injected population (%SP; %DP)3-150 . | Transplanted animal3-151 . | Immunophenotype of tumor in transplanted animals . | ||
|---|---|---|---|---|---|
| %CD8+ . | %CD4+CD8+ . | %CD4+ . | |||
| 71-18 | Unsorted (59%; 40%) | Nu18 | 61 | 35 | 0.4 |
| Nu18 | Unsorted (61%; 35%) | Nu18/1 Nu18/2 Nu18/3 | 32 54 44 | 53 26 35 | 1.7 1.5 0.3 |
| Enriched SP (90%; 8%) | Nu18/SP1 Nu18/SP2 Nu18/SP3 Nu18/SP4 | 21 40 61 45 | 71 47 30 42.5 | 2 1.3 0.5 1 | |
| Enriched DP (2%; 97%) | Nu18/DP1 Nu18/DP2 | 41.5 38 | 47 57 | 2 1.5 | |
| 71-24 | Unsorted (23%; 70%) | Nu24 | 83 | 7 | 0.2 |
| Enriched SP (98%; 1%) | Nu/SP24 | 63 | 30 | 0.4 | |
| Nu24 | Unsorted (83%; 7%) | Nu24/1 Nu24/2 | 69 67 | 15 23 | 0.5 0 |
| Enriched SP (90%; 1%) | Nu24/SP1 Nu24/SP2 Nu24/SP3 | 75 80 72 | 15 16 25 | 0.3 0.5 0 | |
| Enriched DP (15%; 79%) | Nu24/DP1 Nu24/DP2 | 67 60 | 30 35 | 0.4 0.5 | |
| 71-43 | Unsorted (63%; 26%) | Nu43/1 Nu43/2 | 43 39 | 56 58 | 0.3 0.6 |
| Enriched SP (96%; 2%) | Nu/SP43-1 | 66 | 33 | 0.2 | |
Thymic cells from diseased transgenic animals 71-24 and 71-43 and tumor cells developed in nu/nu mice from the leukemic cells of animals 71-18 and 71-24 were sorted according to anti-CD4 expression as DP and CD8+ SP cells (see “Materials and methods”). About 5 × 106 to 10 × 106 of either unsorted or sorted cells were injected subcutaneously in nu/nu mice. The original leukemic cells and tumors developing in primary and secondary recipients were analyzed for CD4 and CD8 expression by flow cytometry.
Percentage of CD8+ SP cells and DP cells in the donor cell population.
Host nu/nu mice injected with either unsorted cells or enriched CD8+ SP or DP cells.