Cell surface marker analysis of leukemic cells obtained from thymus, spleen, and bone marrow of TEL-JAK2 transgenic animals
| Thymus . | Mice . | CD4+ . | CD8+ . | CD4+/CD8+ . |
|---|---|---|---|---|
| 60T1 (control) | 28% | 9% | 59% | |
| 60 | 3.5% | 36% | 54% | |
| 65 | 9% | 14.5% | 67% | |
| 71-2 (control) | 15% | 10% | 59% | |
| 71-4 | 3% | 52% | 40% | |
| 71-11 | 11% | 30% | 50% | |
| 71-24 | 4% | 65% | 18.5% |
| Thymus . | Mice . | CD4+ . | CD8+ . | CD4+/CD8+ . |
|---|---|---|---|---|
| 60T1 (control) | 28% | 9% | 59% | |
| 60 | 3.5% | 36% | 54% | |
| 65 | 9% | 14.5% | 67% | |
| 71-2 (control) | 15% | 10% | 59% | |
| 71-4 | 3% | 52% | 40% | |
| 71-11 | 11% | 30% | 50% | |
| 71-24 | 4% | 65% | 18.5% |
| Spleen . | Mice . | Thy1.2+ . | CD4+ . | CD8+ . | CD4+/CD8+ . |
|---|---|---|---|---|---|
| 3-1-4 (control) | 40% | ND | ND | ND | |
| 3-1-8 | 60% | ND | ND | ND | |
| 60T1 (control) | 19% | 11.5% | 5% | 1.5% | |
| 60 | 75% | 3.5% | 51% | 15% | |
| 65 | 37% | 3.5% | 21% | 8% | |
| 71-2 (control) | 24% | 14% | 8% | 0.6% | |
| 71-4 | 68% | 15% | 36.5% | 22% | |
| Bone Marrow | Mice | Thy1.2+ | CD4+ | CD8+ | CD4+/CD8+ |
| 3-1-4 (control) | 5% | 2% | 2% | 0% | |
| 3-1-8 | 21% | 3.5% | 17% | 0% | |
| 60T1 (control) | 5% | 3% | 0% | 0.5% | |
| 60 | 14% | 5% | 2% | 8% | |
| 65 | 12% | 4% | 2% | 2% | |
| 71-2 (control) | 2.7% | 4% | 1.6% | 0% | |
| 71-4 | 21% | 6% | 6% | 13% |
| Spleen . | Mice . | Thy1.2+ . | CD4+ . | CD8+ . | CD4+/CD8+ . |
|---|---|---|---|---|---|
| 3-1-4 (control) | 40% | ND | ND | ND | |
| 3-1-8 | 60% | ND | ND | ND | |
| 60T1 (control) | 19% | 11.5% | 5% | 1.5% | |
| 60 | 75% | 3.5% | 51% | 15% | |
| 65 | 37% | 3.5% | 21% | 8% | |
| 71-2 (control) | 24% | 14% | 8% | 0.6% | |
| 71-4 | 68% | 15% | 36.5% | 22% | |
| Bone Marrow | Mice | Thy1.2+ | CD4+ | CD8+ | CD4+/CD8+ |
| 3-1-4 (control) | 5% | 2% | 2% | 0% | |
| 3-1-8 | 21% | 3.5% | 17% | 0% | |
| 60T1 (control) | 5% | 3% | 0% | 0.5% | |
| 60 | 14% | 5% | 2% | 8% | |
| 65 | 12% | 4% | 2% | 2% | |
| 71-2 (control) | 2.7% | 4% | 1.6% | 0% | |
| 71-4 | 21% | 6% | 6% | 13% |
Single-cell suspensions were obtained from the indicated organs of founder mice 60 and 65, F1 transgenic mice 71-4, 71-11, and 71-24, and F2 transgenic mouse 3-1-8 and analyzed by flow cytometry for the expression of the indicated markers. Age-matched nontransgenic littermate controls are shown in italics. ND indicates no data.