Conjugate binding assays
Targets . | Effectors . | |
---|---|---|
Donor 1 . | Donor 2 . | |
COL | 25 ± 2.1 | 27 ± 2.3 |
COL + PWM | 21 ± 2.7 | 25 ± 5.5 |
MER | 22 ± 2.4 | 22 ± 3.0 |
MER + PWM | 20 ± 4.2 | 23 ± 4.0 |
Targets . | Effectors . | |
---|---|---|
Donor 1 . | Donor 2 . | |
COL | 25 ± 2.1 | 27 ± 2.3 |
COL + PWM | 21 ± 2.7 | 25 ± 5.5 |
MER | 22 ± 2.4 | 22 ± 3.0 |
MER + PWM | 20 ± 4.2 | 23 ± 4.0 |
PWM treatment of myeloma cells did not result in increased binding to effectors. Myeloma cell lines COL and MER were used as targets. IL-2–activated PBMC effectors from 2 healthy donors were labeled with the fluorescent lipophilic dye PKH26. The E:T-cell ratio was 1:1. Effectors and targets were allowed to bind for 5 to 10 minutes at 37°C prior to counting.
These data represent the percentage binding ± SEM for 3 individual experiments with each donor. At least 1000 effectors were counted for each E:T combination. The differences were not statistically significant.