Table 2.

Conjugate binding assays

TargetsEffectors
Donor 1 Donor 2
COL 25 ± 2.1  27 ± 2.3  
COL + PWM  21 ± 2.7 25 ± 5.5  
MER  22 ± 2.4  22 ± 3.0 
MER + PWM  20 ± 4.2  23 ± 4.0 
TargetsEffectors
Donor 1 Donor 2
COL 25 ± 2.1  27 ± 2.3  
COL + PWM  21 ± 2.7 25 ± 5.5  
MER  22 ± 2.4  22 ± 3.0 
MER + PWM  20 ± 4.2  23 ± 4.0 

PWM treatment of myeloma cells did not result in increased binding to effectors. Myeloma cell lines COL and MER were used as targets. IL-2–activated PBMC effectors from 2 healthy donors were labeled with the fluorescent lipophilic dye PKH26. The E:T-cell ratio was 1:1. Effectors and targets were allowed to bind for 5 to 10 minutes at 37°C prior to counting.

These data represent the percentage binding ± SEM for 3 individual experiments with each donor. At least 1000 effectors were counted for each E:T combination. The differences were not statistically significant.

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