Intraclonal variation of lymphomaVH gene sequences
| Case no. . | Sequences analyzed (no.) . | Noncommon mutations* . | ||
|---|---|---|---|---|
| Total (no.) . | Frequency (%) . | Distribution . | ||
| 1 | 3 | 3 | 0.34 | (1-2/3), (1-1/3) × 2 |
| 2 | 4 | 3 | 0.26 | (3-2/4) |
| 3 | 3 | 0 | 0 | |
| 4 | 3 | 5 | 0.67 | (1-2/3), (1-1/3) × 4 |
| 5 | 2 | 1 | 0.17 | (1-1/2) |
| 6 | 3 | 1 | 0.11 | (1-1/2) |
| 7 | 2 | 0 | 0 | |
| 8 | not cloned | |||
| 9 | 4 | 1 | 0.09 | (1-3/4) |
| 10 | 3 | 1 | 0.11 | (1-1/3) |
| 11 | 3 | 0 | 0 | |
| 12 | 4 | 12 | 0.94 | (4-3/4), (1-2/4), (1-1/4) × 7 |
| 13 | 2 | 0 | 0 | |
| 14 | 5 | 9 | 0.61 | (3-3/5), (4-2/5), (1-1/5) × 2 |
| Case no. . | Sequences analyzed (no.) . | Noncommon mutations* . | ||
|---|---|---|---|---|
| Total (no.) . | Frequency (%) . | Distribution . | ||
| 1 | 3 | 3 | 0.34 | (1-2/3), (1-1/3) × 2 |
| 2 | 4 | 3 | 0.26 | (3-2/4) |
| 3 | 3 | 0 | 0 | |
| 4 | 3 | 5 | 0.67 | (1-2/3), (1-1/3) × 4 |
| 5 | 2 | 1 | 0.17 | (1-1/2) |
| 6 | 3 | 1 | 0.11 | (1-1/2) |
| 7 | 2 | 0 | 0 | |
| 8 | not cloned | |||
| 9 | 4 | 1 | 0.09 | (1-3/4) |
| 10 | 3 | 1 | 0.11 | (1-1/3) |
| 11 | 3 | 0 | 0 | |
| 12 | 4 | 12 | 0.94 | (4-3/4), (1-2/4), (1-1/4) × 7 |
| 13 | 2 | 0 | 0 | |
| 14 | 5 | 9 | 0.61 | (3-3/5), (4-2/5), (1-1/5) × 2 |
Noncommon mutations represent single nucleotide sequence differences identified in only a proportion of theVH clones analyzed. The column labeled “distribution” indicates the number ofVH sequences that contained the noncommon mutation, ie, (2-3/5) indicates that 2 of the noncommon mutations were identified in 3 of 5 VH clones analyzed. Noncommon mutations found in more than half of theVH clones were included in the consensus sequences.