Primitive progenitors in AdGFP-infected subsets
| Sort no. . | Transduction efficiency* . | Population† . | Week 5 . | ||
|---|---|---|---|---|---|
| CFU-Cs‡ . | CAFCs‡ . | Ratio CFU-Cs/ CAFCs . | |||
| 2 | 47.42% | AdGFP | 1509 ± 132 | 848 ± 74 | 1.7 |
| GFP− | 959 ± 114 | 464 ± 98 | 2.0 | ||
| GFP+ | 270 ± 56 | 214 ± 30 | 1.2 | ||
| Mock | 2462 ± 474 | 973 ± 173 | 2.5 | ||
| 4 | 50.9% | AdGFP | 912 ± 94 | 707 ± 109 | 1.3 |
| GFP− | 1097 ± 179 | 439 ± 32 | 2.5 | ||
| GFP+ | 135 ± 22 | 95 ± 4.5 | 1.4 | ||
| Mock | 1335 ± 400 | 627 ± 76 | 2.2 | ||
| 5 | 68.5% | AdGFP | NR | 80 ± 36 | NR |
| GFP− | 48 ± 28 | 95 ± 16 | 0.5 | ||
| GFP+ | 17 ± 7 | 52 ± 21 | 0.3 | ||
| Mock | 394 ± 138 | 199 ± 28 | 2.0 | ||
| 6 | 55.7% | AdGFP | 756 ± 442 | 704 ± 160 | 1.1 |
| GFP− | NR | 584 ± 35 | NR | ||
| GFP+ | 167 ± 52 | 217 ± 14 | 0.8 | ||
| Mock | NR | 954.7 ± 124 | NR | ||
| 7 | 45.8% | AdGFP | 717 ± 16 | 1767 ± 142 | 0.4 |
| GFP− | 1443 ± 274 | 1219 ± 334 | 1.1 | ||
| GFP+ | NR | 543 ± 39 | NR | ||
| Mock | 1459 ± 204 | 1823 ± 389 | 0.8 | ||
| Sort no. . | Transduction efficiency* . | Population† . | Week 5 . | ||
|---|---|---|---|---|---|
| CFU-Cs‡ . | CAFCs‡ . | Ratio CFU-Cs/ CAFCs . | |||
| 2 | 47.42% | AdGFP | 1509 ± 132 | 848 ± 74 | 1.7 |
| GFP− | 959 ± 114 | 464 ± 98 | 2.0 | ||
| GFP+ | 270 ± 56 | 214 ± 30 | 1.2 | ||
| Mock | 2462 ± 474 | 973 ± 173 | 2.5 | ||
| 4 | 50.9% | AdGFP | 912 ± 94 | 707 ± 109 | 1.3 |
| GFP− | 1097 ± 179 | 439 ± 32 | 2.5 | ||
| GFP+ | 135 ± 22 | 95 ± 4.5 | 1.4 | ||
| Mock | 1335 ± 400 | 627 ± 76 | 2.2 | ||
| 5 | 68.5% | AdGFP | NR | 80 ± 36 | NR |
| GFP− | 48 ± 28 | 95 ± 16 | 0.5 | ||
| GFP+ | 17 ± 7 | 52 ± 21 | 0.3 | ||
| Mock | 394 ± 138 | 199 ± 28 | 2.0 | ||
| 6 | 55.7% | AdGFP | 756 ± 442 | 704 ± 160 | 1.1 |
| GFP− | NR | 584 ± 35 | NR | ||
| GFP+ | 167 ± 52 | 217 ± 14 | 0.8 | ||
| Mock | NR | 954.7 ± 124 | NR | ||
| 7 | 45.8% | AdGFP | 717 ± 16 | 1767 ± 142 | 0.4 |
| GFP− | 1443 ± 274 | 1219 ± 334 | 1.1 | ||
| GFP+ | NR | 543 ± 39 | NR | ||
| Mock | 1459 ± 204 | 1823 ± 389 | 0.8 | ||
NR indicates no result.
Percentage of GFP+ in the transduced, unsorted sample.
CD34+ progenitor cells were infected with AdGFP at an MOI of 2000 and sorted into GFP+ and GFP−fractions by flow cytometry. Mock-infected cells and an aliquot of infected cells that was not sorted (AdGFP) served as controls.
Values for GFP+ and GFP− are calculated from week-5 CFU-C or CAFC frequency per 100 000 input cells at week 5 and transduction efficiency.