CFU-C contained in AdGFP-infected subpopulations
| Sort no. . | Transduction efficiency* . | Population† . | GEMM . | Total colonies/100 000 input cells‡ . | Total . | |
|---|---|---|---|---|---|---|
| GM . | Erythroid . | |||||
| 1 | 54.4% | AdGFP | 1100 ± 67 | 1600 ± 267 | 18 167 ± 1022 | 20 867 ± 1111 |
| GFP− | 608 ± 132 | 426 ± 132 | 7205 ± 517 | 8239 ± 780 | ||
| GFP+ | 462 ± 136 | 435 ± 109 | 4162 ± 462 | 5059 ± 490 | ||
| Mock | 1000 ± 333 | 1067 ± 311 | 1533 ± 844 | 17 400 ± 1200 | ||
| 2 | 47.4% | AdGFP | 9033 ± 556 | 4883 ± 511 | 5800 ± 1133 | 19 717 ± 2089 |
| GFP+ | 2486 ± 448 | 1495 ± 280 | 3595 ± 157 | 7567 ± 767 | ||
| Mock | 9333 ± 1844 | 3317 ± 111 | 4800 ± 333 | 17 450 ± 1533 | ||
| 3 | 50.8% | AdGFP | 352 ± 0 | 939 ± 98 | 396 ± 117 | 1687 ± 215 |
| GFP− | 117 ± 17 | 787 ± 84 | 201 ± 0 | 1105 ± 67 | ||
| GFP+ | 86 ± 14 | 280 ± 29 | 162 ± 43 | 528 ± 29 | ||
| 4 | 50.9% | AdGFP | 2500 ± 200 | 7433 ± 356 | 16 500 ± 133 | 26 433 ± 1244 |
| GFP− | 1257 ± 174 | 4459 ± 392 | 9522 ± 152 | 15 239 ± 359 | ||
| GFP+ | 408 ± 170 | 1581 ± 340 | 7089 ± 272 | 9078 ± 612 | ||
| Mock | 2500 ± 800 | 7333 ± 178 | 14 533 ± 2756 | 24 366 ± 2111 | ||
| 5 | 68.5% | AdGFP | 533 ± 111 | 5500 ± 267 | 4133 ± 311 | 10 166 ± 311 |
| GFP− | 144 ± 14 | 3348 ± 124 | 1715 ± 117 | 5208 ± 227 | ||
| GFP+ | 437 ± 61 | 3818 ± 307 | 4508 ± 153 | 8763 ± 368 | ||
| Mock | 1383 ± 211 | 5000 ± 400 | 11 533 ± 3911 | 17 916 ± 3622 | ||
| 6 | 55.7% | AdGFP | 11 950 ± 767 | 6216 ± 244 | 8616 ± 478 | 26 783 ± 411 |
| GFP− | 6718 ± 374 | 2510 ± 521 | 3307 ± 452 | 12 138 ± 487 | ||
| GFP+ | 4753 ± 87 | 2692 ± 321 | 6888 ± 173 | 14 333 ± 360 | ||
| Mock | 10 316 ± 778 | 5775 ± 350 | 12 333 ± 1089 | 28 425 ± 2033 | ||
| Sort no. . | Transduction efficiency* . | Population† . | GEMM . | Total colonies/100 000 input cells‡ . | Total . | |
|---|---|---|---|---|---|---|
| GM . | Erythroid . | |||||
| 1 | 54.4% | AdGFP | 1100 ± 67 | 1600 ± 267 | 18 167 ± 1022 | 20 867 ± 1111 |
| GFP− | 608 ± 132 | 426 ± 132 | 7205 ± 517 | 8239 ± 780 | ||
| GFP+ | 462 ± 136 | 435 ± 109 | 4162 ± 462 | 5059 ± 490 | ||
| Mock | 1000 ± 333 | 1067 ± 311 | 1533 ± 844 | 17 400 ± 1200 | ||
| 2 | 47.4% | AdGFP | 9033 ± 556 | 4883 ± 511 | 5800 ± 1133 | 19 717 ± 2089 |
| GFP+ | 2486 ± 448 | 1495 ± 280 | 3595 ± 157 | 7567 ± 767 | ||
| Mock | 9333 ± 1844 | 3317 ± 111 | 4800 ± 333 | 17 450 ± 1533 | ||
| 3 | 50.8% | AdGFP | 352 ± 0 | 939 ± 98 | 396 ± 117 | 1687 ± 215 |
| GFP− | 117 ± 17 | 787 ± 84 | 201 ± 0 | 1105 ± 67 | ||
| GFP+ | 86 ± 14 | 280 ± 29 | 162 ± 43 | 528 ± 29 | ||
| 4 | 50.9% | AdGFP | 2500 ± 200 | 7433 ± 356 | 16 500 ± 133 | 26 433 ± 1244 |
| GFP− | 1257 ± 174 | 4459 ± 392 | 9522 ± 152 | 15 239 ± 359 | ||
| GFP+ | 408 ± 170 | 1581 ± 340 | 7089 ± 272 | 9078 ± 612 | ||
| Mock | 2500 ± 800 | 7333 ± 178 | 14 533 ± 2756 | 24 366 ± 2111 | ||
| 5 | 68.5% | AdGFP | 533 ± 111 | 5500 ± 267 | 4133 ± 311 | 10 166 ± 311 |
| GFP− | 144 ± 14 | 3348 ± 124 | 1715 ± 117 | 5208 ± 227 | ||
| GFP+ | 437 ± 61 | 3818 ± 307 | 4508 ± 153 | 8763 ± 368 | ||
| Mock | 1383 ± 211 | 5000 ± 400 | 11 533 ± 3911 | 17 916 ± 3622 | ||
| 6 | 55.7% | AdGFP | 11 950 ± 767 | 6216 ± 244 | 8616 ± 478 | 26 783 ± 411 |
| GFP− | 6718 ± 374 | 2510 ± 521 | 3307 ± 452 | 12 138 ± 487 | ||
| GFP+ | 4753 ± 87 | 2692 ± 321 | 6888 ± 173 | 14 333 ± 360 | ||
| Mock | 10 316 ± 778 | 5775 ± 350 | 12 333 ± 1089 | 28 425 ± 2033 | ||
Percentage of GFP+ in the transduced, unsorted sample.
CD34+ progenitor cells were infected with AdGFP at an MOI of 2000 and sorted into GFP+ and GFP−fractions by flow cytometry. Mock-infected cells and an aliquot of infected cells that was not sorted (AdGFP) served as controls.
Values for GFP+ and GFP− fractions are absolute numbers per 100 000 input cells calculated from week-5 CFU-C or week-5 CAFC frequency per 100 000 input cells and initial transduction efficiency.