Competitive repopulation in primary and secondary transplants
| Treatment . | CFU-GEMM . | CFC . | Pre-B CFU . | |||
|---|---|---|---|---|---|---|
| PBS . | Epo . | PBS . | Epo . | PBS . | Epo . | |
| Primary recipients | 64 ± 9.5% (119) | 100 ± 0% (89) | 49 ± 7.3% (72) | 100 ± 0% (109) | 52 ± 9.6% (204) | 84 ± 8.3% (153) |
| Secondary recipients | 71 ± 14% (28) | 96 ± 3.5% (25) | 70 ± 10% (35) | 86 ± 4.0% (20) | 71 ± 5.0% (24) | 86 ± 4.0% (20) |
| Treatment . | CFU-GEMM . | CFC . | Pre-B CFU . | |||
|---|---|---|---|---|---|---|
| PBS . | Epo . | PBS . | Epo . | PBS . | Epo . | |
| Primary recipients | 64 ± 9.5% (119) | 100 ± 0% (89) | 49 ± 7.3% (72) | 100 ± 0% (109) | 52 ± 9.6% (204) | 84 ± 8.3% (153) |
| Secondary recipients | 71 ± 14% (28) | 96 ± 3.5% (25) | 70 ± 10% (35) | 86 ± 4.0% (20) | 71 ± 5.0% (24) | 86 ± 4.0% (20) |
Percentages are the mean % ± SEM of transgene-bearing colonies detected in BM harvested from animals 6 months after primary and secondary transplants. Numbers in parentheses represent total colonies of each class analyzed. There were a total of 11 to 15 different animals for each group (PBS and epo-treated) in the primary transplants and 2 animals for each group in the secondary transplants. P ≤ .05 for each type of CFU when percentage of cells of transgene origin in epo-treated primary recipients is compared to the percentage in PBS-treated animals, and <.001 when compared to the input ratio (50/50). P values for percent transgene-bearing CFU in the secondary transplant epo-treated animals when compared to secondary PBS-treated animals were .15, .038, and .18 (GEMM, CFC, pre-B) and .018, .28, .061 when compared to the percent transgene-bearing CFU in the primary PBS-treated donor animals.
Abbreviations are as in Table 1.