MCP-1 has no effect on the in vitro growth of MDA-231 cells
| MCP-1 (ng/mL) . | 3H-thymidine incorporation (CPM) . | |
|---|---|---|
| No antibody . | Anti-MCP-1 (10 μg/mL) . | |
| 0 | 45 260 (±4011) | 48 269 (±2300) |
| 1 | 41 670 (±3999) | 44 500 (±1890) |
| 10 | 44 380 (±3457) | 43 012 (±3889) |
| 100 | 48 000 (±7001) | 43 991 (±5012) |
| MCP-1 (ng/mL) . | 3H-thymidine incorporation (CPM) . | |
|---|---|---|
| No antibody . | Anti-MCP-1 (10 μg/mL) . | |
| 0 | 45 260 (±4011) | 48 269 (±2300) |
| 1 | 41 670 (±3999) | 44 500 (±1890) |
| 10 | 44 380 (±3457) | 43 012 (±3889) |
| 100 | 48 000 (±7001) | 43 991 (±5012) |
The proliferation of MDA-231 cells was tested in the presence of different concentrations of exogenous MCP-1 and compared with that observed in the presence of medium alone. The effect of an MCP-1 antibody on MDA-231 cellular proliferation was also tested, as measured by 3H-thymidine incorporation after 48 hours of MCP-1 stimulation. Each MCP-1 concentration was tested in triplicate. The average results of 3 experiments are shown ± SEM. Statistical analysis was performed, using analysis of variance relative to the medium alone.