MCP-1 induces rat endothelial cell sprouting in a dose-dependent manner
| Experiment number . | Aortic rings . | MCP-1 concentration (ng/mL) . | ECGS (100 ng/mL) . | ||||
|---|---|---|---|---|---|---|---|
| 0 . | 0.5 . | 5 . | 50 . | 500 . | |||
| 1 | 1 | — | — | + | ++ | + | +++ |
| 2 | — | ++ | ++ | +++ | + | + | |
| 3 | — | + | — | ++ | + | ++ | |
| 4 | — | + | — | + | — | + | |
| 5 | — | + | ++ | + | — | + | |
| 6 | — | — | + | — | — | — | |
| 2 | 1 | + | ++ | + | ++ | ++ | ++++ |
| 2 | — | + | ++ | +++ | + | +++ | |
| 3 | + | — | — | +++ | — | ++++ | |
| 4 | — | — | + | ++ | — | ++ | |
| 5 | — | + | ++ | + | — | ++++ | |
| 6 | — | — | — | — | — | ++ | |
| Experiment number . | Aortic rings . | MCP-1 concentration (ng/mL) . | ECGS (100 ng/mL) . | ||||
|---|---|---|---|---|---|---|---|
| 0 . | 0.5 . | 5 . | 50 . | 500 . | |||
| 1 | 1 | — | — | + | ++ | + | +++ |
| 2 | — | ++ | ++ | +++ | + | + | |
| 3 | — | + | — | ++ | + | ++ | |
| 4 | — | + | — | + | — | + | |
| 5 | — | + | ++ | + | — | + | |
| 6 | — | — | + | — | — | — | |
| 2 | 1 | + | ++ | + | ++ | ++ | ++++ |
| 2 | — | + | ++ | +++ | + | +++ | |
| 3 | + | — | — | +++ | — | ++++ | |
| 4 | — | — | + | ++ | — | ++ | |
| 5 | — | + | ++ | + | — | ++++ | |
| 6 | — | — | — | — | — | ++ | |
Induction of endothelial cell sprouting by different concentrations of MCP-1 was compared with that observed in the presence of medium alone (negative control) or ECGS (positive control). Each concentration was tested by sextuples, and the aortic rings were scored as follows: —, no sprouting; +, low sprouting levels; ++, 25%-50% sprouting; +++, 50%-75% sprouting; ++++, more than 75% sprouting. The scores of 2 separate experiments are shown. ECGS, endothelial cell growth supplement.