Tubular necrosis, neutrophil accumulation, and congestion in the outer medulla in rats subjected to renal ischemia followed by reperfusion
| Treatment Group . | Tubular Necrosis (%) . | Neutrophils (/HPF) . | Erythrocytes (/HPF) . |
|---|---|---|---|
| Sham operation | 0.0 ± 0.0 | 0.1 ± 0.1 | 80.3 ± 17.1 |
| I/R + saline | 84.8 ± 9.1* | 8.3 ± 3.7* | 352.7 ± 48.1* |
| I/R + APC | 51.4 ± 11.8† | 1.9 ± 1.6† | 183.6 ± 65.9† |
| I/R + DEGR-FXa | 82.0 ± 6.8* | 8.2 ± 3.1* | 343.2 ± 67.3* |
| I/R + heparin | 81.6 ± 7.5* | 7.8 ± 2.6* | 304.4 ± 44.6* |
| I/R + DIP-APC | 80.0 ± 8.7* | 8.3 ± 3.3* | 345.6 ± 70.2* |
| I/R + leukocytopenia | 32.6 ± 12.9† | 0.8 ± 0.7† | 175.8 ± 80.4† |
| Treatment Group . | Tubular Necrosis (%) . | Neutrophils (/HPF) . | Erythrocytes (/HPF) . |
|---|---|---|---|
| Sham operation | 0.0 ± 0.0 | 0.1 ± 0.1 | 80.3 ± 17.1 |
| I/R + saline | 84.8 ± 9.1* | 8.3 ± 3.7* | 352.7 ± 48.1* |
| I/R + APC | 51.4 ± 11.8† | 1.9 ± 1.6† | 183.6 ± 65.9† |
| I/R + DEGR-FXa | 82.0 ± 6.8* | 8.2 ± 3.1* | 343.2 ± 67.3* |
| I/R + heparin | 81.6 ± 7.5* | 7.8 ± 2.6* | 304.4 ± 44.6* |
| I/R + DIP-APC | 80.0 ± 8.7* | 8.3 ± 3.3* | 345.6 ± 70.2* |
| I/R + leukocytopenia | 32.6 ± 12.9† | 0.8 ± 0.7† | 175.8 ± 80.4† |
Rats were subjected to 60 minutes of renal ischemia followed by reperfusion. Kidney samples were obtained 24 hours after reperfusion. Data are expressed as mean ± SD values from 5 experiments. HPF indicates high-power field; I/R, ischemia/reperfusion; APC, activated protein C; DEGR-FXa, dansyl glutamylglycylarginyl chloromethyl ketone–treated factor Xa; and DIP-APC, diisopropyl fluorophosphate–treated APC.
P < .01 compared with sham operation group.
P < .01 compared with I/R + saline group.