Effect of different mutations of Shc on binding to SHIP and Grb2
| Shc Mutant Form . | SHIP . | Grb2 . |
|---|---|---|
| Wild-type | ++++ | ++++ |
| R175M | − | − |
| R401M | ++++ | ++++ |
| 2M | − | − |
| Y239F | ++++ | +++ |
| Y240F | ++++ | ++++ |
| 2F | +/− | ++ |
| Y317F | +/− | + |
| 3F | − | − |
| Shc Mutant Form . | SHIP . | Grb2 . |
|---|---|---|
| Wild-type | ++++ | ++++ |
| R175M | − | − |
| R401M | ++++ | ++++ |
| 2M | − | − |
| Y239F | ++++ | +++ |
| Y240F | ++++ | ++++ |
| 2F | +/− | ++ |
| Y317F | +/− | + |
| 3F | − | − |
SHIP indicates SH2-containing 5′ inositol phosphatase; ++++, strong binding; +++ or ++, moderate binding; + or +/−, weak binding; and a dash, no binding. Densitometry analysis was performed on all blots shown in Figure 4. Results are relative to those with wild-type Shc cells. Background was determined from the vector-transfected cells.