Effect of transforming growth factor β in different cytokine cocktails
| . | . | ESI . | ES . | EI . | SI . |
|---|---|---|---|---|---|
| Proportion F+ cells | CON | 30 | 20 | 34 | 18 |
| (percentage of Hb+) | TGF | 76 | 75 | 72 | 65 |
| Proportion F+A− cells | CON | 9.4 | 5.6 | 14 | 7.8 |
| (percentage of F+) | TGF | 39 | 47 | 41 | 29 |
| Relative TGF effect | F+ | 1.15 | 1.15 | 0.65 | 1.05 |
| (N[TGF]/N[CON]) | F−A+ | 0.14 | 0.08 | 0.13 | 0.12 |
| Secondary colonies | CON | 100 | 26 | 38 | 74 |
| (normalized) | TGF | 61 | 18 | 19 | 39 |
| . | . | ESI . | ES . | EI . | SI . |
|---|---|---|---|---|---|
| Proportion F+ cells | CON | 30 | 20 | 34 | 18 |
| (percentage of Hb+) | TGF | 76 | 75 | 72 | 65 |
| Proportion F+A− cells | CON | 9.4 | 5.6 | 14 | 7.8 |
| (percentage of F+) | TGF | 39 | 47 | 41 | 29 |
| Relative TGF effect | F+ | 1.15 | 1.15 | 0.65 | 1.05 |
| (N[TGF]/N[CON]) | F−A+ | 0.14 | 0.08 | 0.13 | 0.12 |
| Secondary colonies | CON | 100 | 26 | 38 | 74 |
| (normalized) | TGF | 61 | 18 | 19 | 39 |
Cultures were initiated in media with different combinations of cytokines in the presence or absence of TGFβ. After 4 days, all cultures were reseeded without TGFβ in fresh medium with the full complement of SCF, IL3, and EPO. Flow cytometric analysis on day 8 of culture. Secondary colonies were counted on day 14 and normalized to 100 for the control cultures in EPO, SCF, and IL3. Data represent the averaged values from 2 independent experiments with blood from different donors. Numerical results from both experiments were all within 10% of each other, so that the display of ranges is omitted for simplicity.
ESI = EPO + SCF + IL3; ES = EPO + SCF; EI = EPO + IL3; SI = SCF + IL3.