The effect of TOR on the production of latent TGF-β1 by adherent PBMC cultured with IL-4 + GM-CSF
| Treatment . | Day 1 . | Day 3 . | Day 7 . |
|---|---|---|---|
| Control | 1.1 ± 0.18 | 1.1 ± 0.11 | 1.2 ± 0.43 |
| IL-4 + GM-CSF | 1.2 ± 0.24 | 1.2 ± 0.24 | 1.2 ± 0.43 |
| +TOR 10 μmol/L | 1.2 ± 0.21 | 1.2 ± 0.18 | 1.2 ± 0.51 |
| Treatment . | Day 1 . | Day 3 . | Day 7 . |
|---|---|---|---|
| Control | 1.1 ± 0.18 | 1.1 ± 0.11 | 1.2 ± 0.43 |
| IL-4 + GM-CSF | 1.2 ± 0.24 | 1.2 ± 0.24 | 1.2 ± 0.43 |
| +TOR 10 μmol/L | 1.2 ± 0.21 | 1.2 ± 0.18 | 1.2 ± 0.51 |
Adherent PBMC were cultured in a medium alone (control) or with IL-4 (500 U/mL) + GM-CSF (50 ng/mL) in the presence or absence of TOR. After 1, 3, and 7 days the latent TGF-β1 levels were measured from the culture supernatants by ELISA. The data represent TGF-β1 levels (ng/mL) of 4 individual experiments (mean ± SD). The amount of latent TGF-β1 in the culture medium without cells added was 0.9 ng/mL. The data were similar for 1-5 μmol/L TOR concentrations.